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Literature summary for 4.1.2.21 extracted from

  • Ran, N.; Frost, J.W.
    Directed evolution of 2-keto-3-deoxy-6-phosphogalactonate aldolase to replace 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase (2007), J. Am. Chem. Soc., 129, 6130-6139.
    View publication on PubMed
Search for more literature for 4.1.2.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain NR7 Escherichia coli

Protein Variants

Protein Variants Comment Organism
F33I/D58N/Q72H/A75V/V85A/V154F higher activity compared to the wild type enzyme Escherichia coli
additional information mutant NR8.276-2 exhibits a 60fold improvement in the ratio kcat/KM relative to that of the wild type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 D-erythrose 4-phosphate mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli
0.57
-
 D-erythrose 4-phosphate wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 4B resin column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 Escherichia coli pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
pyruvate + D-erythrose 4-phosphate
-
 Escherichia coli 3-deoxy-D-arabino-heptulosonic acid 7-phosphate
-
 r

Synonyms

Synonyms Comment Organism
2-keto-3-deoxy-6-phosphogalactonate aldolase
-
 Escherichia coli
DgoA
-
 Escherichia coli
KDPGal aldolase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.94
-
 D-erythrose 4-phosphate wild type enzyme, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli
2.5
-
 D-erythrose 4-phosphate mutant enzyme F33I/D58N/Q72H/A75V/V85A/V154F, in MOPS buffer (50 mM, pH 7.5), 0.050 mM CoCl2, 0.010 mM NAD+, D-erythrose 4-phosphate, 2 mM pyruvate, 1 unit of 3-dehydroquinate synthase, 1 unit of 3-dehydroquinate dehydratase, at 25°C Escherichia coli