Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | LiCl | Escherichia coli | |
NaBH4 | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-xylulose 1-phosphate | - |
Escherichia coli | |
0.3 | - |
L-rhamnulose 1-phosphate | - |
Escherichia coli | |
1.8 | - |
D-sorbose 1-phosphate | - |
Escherichia coli | |
3 | - |
glycerone phosphate | - |
Escherichia coli | |
6 | - |
L-lactaldehyde | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
K+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
Na+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
NH4+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
Rb+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.2 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
0-4°C, crystalline enzyme, containing 0.2-1.0% protein, stable for at least 3 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-Deoxy-L-sorbose 1-phosphate | 0.8% of the activity with L-rhamnulose 1-phosphate | Escherichia coli | Glycerone phosphate + D-lactaldehyde | - |
? | |
D-Fructose 1-phosphate | 1.7% of the activity with L-rhanmnulose 1-phosphate | Escherichia coli | ? | - |
? | |
D-Fructose-1,6-diphosphate | 1.8% of the activity with L-rhanmnulose 1-phosphate | Escherichia coli | ? | - |
? | |
D-Ribulose 1,5-diphosphate | 1.3% of the activity with L-rhanmnulose 1-phosphate | Escherichia coli | ? | - |
? | |
Glycerone phosphate + (S)-lactaldehyde | - |
Escherichia coli | L-Rhamnulose 1-phosphate | - |
? | |
Glycerone phosphate + acetaldehyde | at 10% of the activity with L-lactaldehyde | Escherichia coli | 5-Deoxy-L-xylulose 1-phosphate | - |
? | |
glycerone phosphate + D-glyceraldehyde | at 50% of the activity with L-lactaldehyde | Escherichia coli | D-sorbose 1-phosphate | - |
? | |
Glycerone phosphate + D-lactaldehyde | at 10% of the activity with L-lactaldehyde | Escherichia coli | 6-Deoxy-D-sorbose 1-phosphate | - |
? | |
Glycerone phosphate + formaldehyde | at 10% of the activity with L-lactaldehyde | Escherichia coli | D-Erythrulose 1-phosphate | - |
? | |
Glycerone phosphate + glycolaldehyde | at 40% of the activity with L-lactaldehyde | Escherichia coli | L-Xylulose 1-phosphate | - |
? | |
L-Rhamnulose 1-phosphate | - |
Escherichia coli | Glycerone phosphate + (S)-lactaldehyde | - |
? | |
L-Sorbose 1-phosphate | 0.3% of the activity with L-rhanmnulose 1-phosphate | Escherichia coli | Glycerone phosphate + D-glyceraldehyde | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.3 | - |
L-rhamnulose 1-phosphate | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.9: about 50% of maximal activity | Escherichia coli |