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Literature summary for 4.1.2.19 extracted from

  • Chiu, T.H.; Feingold, D.S.
    L-Rhamnulose 1-phosphate aldolase from Escherichia coli. Crystallization and properties (1969), Biochemistry, 8, 98-108.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Li+ LiCl Escherichia coli
NaBH4
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
L-xylulose 1-phosphate
-
Escherichia coli
0.3
-
L-rhamnulose 1-phosphate
-
Escherichia coli
1.8
-
D-sorbose 1-phosphate
-
Escherichia coli
3
-
glycerone phosphate
-
Escherichia coli
6
-
L-lactaldehyde
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
K+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
Na+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
NH4+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli
Rb+ Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.2
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
0-4°C, crystalline enzyme, containing 0.2-1.0% protein, stable for at least 3 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-Deoxy-L-sorbose 1-phosphate 0.8% of the activity with L-rhamnulose 1-phosphate Escherichia coli Glycerone phosphate + D-lactaldehyde
-
?
D-Fructose 1-phosphate 1.7% of the activity with L-rhanmnulose 1-phosphate Escherichia coli ?
-
?
D-Fructose-1,6-diphosphate 1.8% of the activity with L-rhanmnulose 1-phosphate Escherichia coli ?
-
?
D-Ribulose 1,5-diphosphate 1.3% of the activity with L-rhanmnulose 1-phosphate Escherichia coli ?
-
?
Glycerone phosphate + (S)-lactaldehyde
-
Escherichia coli L-Rhamnulose 1-phosphate
-
?
Glycerone phosphate + acetaldehyde at 10% of the activity with L-lactaldehyde Escherichia coli 5-Deoxy-L-xylulose 1-phosphate
-
?
glycerone phosphate + D-glyceraldehyde at 50% of the activity with L-lactaldehyde Escherichia coli D-sorbose 1-phosphate
-
?
Glycerone phosphate + D-lactaldehyde at 10% of the activity with L-lactaldehyde Escherichia coli 6-Deoxy-D-sorbose 1-phosphate
-
?
Glycerone phosphate + formaldehyde at 10% of the activity with L-lactaldehyde Escherichia coli D-Erythrulose 1-phosphate
-
?
Glycerone phosphate + glycolaldehyde at 40% of the activity with L-lactaldehyde Escherichia coli L-Xylulose 1-phosphate
-
?
L-Rhamnulose 1-phosphate
-
Escherichia coli Glycerone phosphate + (S)-lactaldehyde
-
?
L-Sorbose 1-phosphate 0.3% of the activity with L-rhanmnulose 1-phosphate Escherichia coli Glycerone phosphate + D-glyceraldehyde
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.3
-
L-rhamnulose 1-phosphate
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 30% of maximal activity, pH 9.9: about 50% of maximal activity Escherichia coli