Cloned (Comment) | Organism |
---|---|
His6-tagged KDPG aldolase is cloned in Escherichia coli BL21 | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
2-keto-3-deoxy-6-phosphogluconate | - |
Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified by using Ni2+ affinity column | Thermotoga maritima |
Storage Stability | Organism |
---|---|
protein can be stored at 4°C for up to 6 months without a significant loss in activity. | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
Thermotoga maritima | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
2-pyridinecarboxyaldehyde + pyruvate | typical isolated yield of product is 80-90% after reverse-phase C18 purification. Reaction is also doable with substrates other than 2-pyridinecarboxyaldehyde. Enantiomeric excess greater than 99.7% | Thermotoga maritima | (S)-4-hydroxy-2-keto-4-(2'-pyridyl)butyrate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxy-6-phosphogluconate aldolase | - |
Thermotoga maritima |
KDPG aldolase | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
2-keto-3-deoxy-6-phosphogluconate | - |
Thermotoga maritima |