Literature summary for 4.1.2.13 extracted from

Boucher, L.E.; Bosch, J.
Structure of Toxoplasma gondii fructose-1,6-bisphosphate aldolase (2014), Acta Crystallogr. Sect. F, 70, 1186-1192 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallized in the presence of glycerol and PEG 4000 at 4°C, producing crystals that are cryoprotected with a cryosolution containing reservoir and glycerol at a final concentration of 35% (v/v) before flash-cooling in liquid nitrogen. Crystallized in space group P22(1)2(1), with the biologically relevant tetramer in the asymmetric unit, and the structure has been determined via molecular replacement to a resolution of 2.0 A	Toxoplasma gondii
sitting drop vapor diffusion method, using 0.1 M MOPS/HEPES-Na pH 7.9, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate, 0.02 M ammonium acetate, 12.5% (v/v) glycerol, 25% (w/v) PEG 4000	Toxoplasma gondii

Crystallization (Comment)

Organism

crystallized in the presence of glycerol and PEG 4000 at 4°C, producing crystals that are cryoprotected with a cryosolution containing reservoir and glycerol at a final concentration of 35% (v/v) before flash-cooling in liquid nitrogen. Crystallized in space group P22(1)2(1), with the biologically relevant tetramer in the asymmetric unit, and the structure has been determined via molecular replacement to a resolution of 2.0 A

Toxoplasma gondii

sitting drop vapor diffusion method, using 0.1 M MOPS/HEPES-Na pH 7.9, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate, 0.02 M ammonium acetate, 12.5% (v/v) glycerol, 25% (w/v) PEG 4000

Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 1,6-bisphosphate	Toxoplasma gondii	-	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 1,6-bisphosphate	Toxoplasma gondii ATCC 50861	-	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Toxoplasma gondii	B9PW35	-	-
Toxoplasma gondii ATCC 50861	B9PW35	-	-

Purification (Commentary)

Purification (Comment)	Organism
Co-TALON column chromatography and Superdex 200 gel filtration	Toxoplasma gondii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 1,6-bisphosphate	-	Toxoplasma gondii	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?
D-fructose 1,6-bisphosphate	-	Toxoplasma gondii ATCC 50861	glycerone phosphate + D-glyceraldehyde 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
fructose-1,6-bisphosphate aldolase	-	Toxoplasma gondii

General Information

General Information	Comment	Organism
physiological function	during invasion of the apicomplexan parasite Toxoplasma gondii into host cells, the enzyme serves in the role of a structural bridging protein, as opposed to its normal enzymatic role in the glycolysis pathway	Toxoplasma gondii