Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3)Star | Giardia intestinalis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with 18-23% polyethylene glycol monomethyl ether 2000, 0.1 M Tris-HCl (pH 8.8), and 0.2 M MgCl2 | Giardia intestinalis |
Protein Variants | Comment | Organism |
---|---|---|
D83A | impaired enzyme activity | Giardia intestinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phosphoglycolohydroxamate | - |
Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 25°C, in the absence of Zn2+ | Giardia intestinalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent, the active site contains a zinc ion | Giardia intestinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35380 | - |
calculated from amino acid sequence | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose Q chromatography, Sephacryl 100 gel filtration, and phenyl-Sepharose chromatography | Giardia intestinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Giardia intestinalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Giardia intestinalis | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
additional information | FBPA exhibits very low activity toward D-tagatose-1,6-bisphosphate | Giardia intestinalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Giardia intestinalis |
Synonyms | Comment | Organism |
---|---|---|
FBPA | - |
Giardia intestinalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.55 | - |
D-fructose 1,6-bisphosphate | at pH 7.5 and 25°C, in the absence of Zn2+ | Giardia intestinalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
Phosphoglycolohydroxamate | at pH 7.5 and 25°C, in the absence of Zn2+ | Giardia intestinalis |