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Literature summary for 4.1.2.13 extracted from

  • Ertunga, N.; Colak, A.; Belduz, A.O.; Canakei, S.; Karaoglu, H.; Sandalli, C.
    Cloning, expression, purification and characterization of fructose-1,6-bisphosphate aldolase from Anoxybacillus gonensis G2 (2007), J. Biochem., 141, 817-825.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Anoxybacillus gonensis

Inhibitors

Inhibitors Comment Organism Structure
Cr3+ 1 mM, 36% decrease of activity Anoxybacillus gonensis
Cu2+ 1 mM, 57% decrease of activity Anoxybacillus gonensis
EDTA 1 mM, complete inhibition Anoxybacillus gonensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.576
-
D-fructose 1,6-bisphosphate in 50 mM Tris-HCl buffer at pH 8.5 and 60°C Anoxybacillus gonensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 82% increase of activity Anoxybacillus gonensis
Co2+ 1 mM, 63% increase of activity Anoxybacillus gonensis
K+ 1 mM, 30% increase of activity Anoxybacillus gonensis
additional information Cd2+ has no effect on activity Anoxybacillus gonensis
Zn2+ 1 mM, 189% increase of activity Anoxybacillus gonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30900
-
calculated from sequence of cDNA Anoxybacillus gonensis

Organism

Organism UniProt Comment Textmining
Anoxybacillus gonensis A1YZ23
-
-
Anoxybacillus gonensis G2 A1YZ23
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity chromatography Anoxybacillus gonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Anoxybacillus gonensis glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?
D-fructose 1,6-bisphosphate
-
Anoxybacillus gonensis G2 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
FBP aldolase
-
Anoxybacillus gonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Anoxybacillus gonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Anoxybacillus gonensis