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Literature summary for 4.1.2.13 extracted from

  • Zgiby, S.; Plater, A.R.; Bates, M.A.; Thomson, G.J.; Berry, A.
    A functional role for a flexible loop containing Glu182 in the class II fructose-1,6-bisphosphate aldolase from Escherichia coli (2002), J. Mol. Biol., 315, 131-140.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E174A severely crippled catalysis Escherichia coli
E181A mutant enzyme has wild-type properties Escherichia coli
E182A decrease in kcat Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 D-fructose 1,6-bisphosphate E182A mutant, 30°C Escherichia coli
0.13
-
 D-fructose 1,6-bisphosphate E174A mutant, 30°C Escherichia coli
0.17
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 30°C Escherichia coli
0.3
-
 D-fructose 1,6-bisphosphate E181A mutant, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
class II enzyme Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
 Escherichia coli glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 D-fructose 1,6-bisphosphate E174A mutant, 30°C Escherichia coli
0.033
-
 D-fructose 1,6-bisphosphate E182A mutant, 30°C Escherichia coli
5.8
-
 D-fructose 1,6-bisphosphate E181A mutant, 30°C Escherichia coli
10.5
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 30°C Escherichia coli