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Literature summary for 4.1.2.13 extracted from
- Fructose-1,6-bisphosphate aldolase from Bacillus subtilis (1982), Methods Enzymol., 90, 235-241.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Bacillus subtilis |  |
| Ca2+ | - |
Bacillus subtilis | |
| Chelators | - |
Bacillus subtilis | |
| oxaloacetate | - |
Bacillus subtilis | |
| pyruvate | - |
Bacillus subtilis | |
| sulfhydryl reagents | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulates | Bacillus subtilis | |
| NH4+ | stimulates | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000, SDS-PAGE | Bacillus subtilis |
| 120000 | - |
sucrose density gradient centrifugation | Bacillus subtilis |
| 150000 | - |
gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
strain PCI 219 and strain 168 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spore | - |
Bacillus subtilis | - |
| vegetative | - |
Bacillus subtilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
strain 168, spore | Bacillus subtilis |
| 8.85 | - |
strain PCI 219, vegetative cell | Bacillus subtilis |
| 13.2 | - |
strain 168, vegetative cell | Bacillus subtilis |
| 32.3 | - |
strain PCI 219, spore | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate | - |
Bacillus subtilis | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| additional information | a trace of activity towards fructose 6-phosphate, deoxyribose 5-phosphate and ribose 5-phosphate | Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 30000, SDS-PAGE | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
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