Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Prunus dulcis |
Protein Variants | Comment | Organism |
---|---|---|
I108M/A111G | mutant with improved catalytic properties: recovery of a 89.9% yield of (R)-2-chloromandelonitrile with an enantiomeric excess of 97.6% enantiomeric excess, about 4% residual aldehyde. The mutant also contains two silent mutations at position 85 and position 432 | Prunus dulcis |
L1Q | mutation enhances expression in Pichia pastoris | Prunus dulcis |
L1Q/A111G | activity is similar to wild-type enzyme with its preferred substrate benzaldehyde. Directed evolution of PaHNL5/L1Q/A11G | Prunus dulcis |
L1Q/N3I/I108M/A111G | mutant with improved catalytic properties: recovery of a 92.7% yield of (R)-2-chloromandelonitrile with an ee of 98.6% enantiomeric excess, about 2% residual aldehyde, the mutant also contains two silent mutations at position 85 and position 432 | Prunus dulcis |
additional information | directed evolution of PaHNL5/L1Q/A11G (a mutant with activity is similar to wild-type enzyme with its preferred substrate benzaldehyde). 10000 transformants are screened for cleavage or synthesis of (R)-2-chloromandelonitrile | Prunus dulcis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanide + benzaldehyde | Prunus dulcis | - |
(R)-mandelonitrile | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prunus dulcis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanide + 2-chlorobenzaldehyde | - |
Prunus dulcis | (R)-2-chloromandelonitrile | - |
r | |
cyanide + benzaldehyde | - |
Prunus dulcis | (R)-mandelonitrile | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(R)-HNL | - |
Prunus dulcis |
HNL | - |
Prunus dulcis |
Hydroxynitrile lyase | - |
Prunus dulcis |
PaHNL5 | isoenzyme 5 | Prunus dulcis |