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Literature summary for 4.1.1.B12 extracted from

  • Jeong, S.; Yeon, Y.; Choi, E.; Byun, S.; Cho, D.; Kim, I.; Kim, Y.
    Alkaliphilic lysine decarboxylases for effective synthesis of cadaverine from L-lysine (2016), Korean J. Chem. Engin., 33, 1530-1533 .
No PubMed abstract available
Search for more literature for 4.1.1.B12

Application

Application Comment Organism
synthesis the enzyme can potentially be used for synthesis and production of cadaverine. Cadaverine is a promising chemical platform that has a variety of applications, including the production of polyamides, polyurethanes, chelating agents, and additives. In particular, it is relevant for the production of bio-based nylon, which can be used to replace conventional polyamides from petrochemical routes. Whereas Nylon 66 is a polymer composed of hexamethylenediamine and adipic acid, Nylon 56 is a polymer that is produced by the co-polymerization of cadaverine and adipic acid Selenomonas ruminantium
synthesis the enzyme can potentially be used for synthesis and production of cadaverine. Cadaverine is a promising chemical platform that has a variety of applications, including the production of polyamides, polyurethanes, chelating agents, and additives. In particular, it is relevant for the production of bio-based nylon, which can be used to replace conventional polyamides from petrochemical routes. Whereas Nylon 66 is a polymer composed of hexamethylenediamine and adipic acid, Nylon 56 is a polymer that is produced by the co-polymerization of cadaverine and adipic acid Vibrio vulnificus

Cloned(Commentary)

Cloned (Comment) Organism
gene srLDC, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Selenomonas ruminantium
gene vvLDC, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Vibrio vulnificus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine Selenomonas ruminantium
-
 cadaverine + CO2
-
 ?
L-lysine Vibrio vulnificus
-
 cadaverine + CO2
-
 ?
L-ornithine Selenomonas ruminantium
-
 putrescine + CO2
-
 ?
L-ornithine Vibrio vulnificus
-
 putrescine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Selenomonas ruminantium O50657
-
-
Vibrio vulnificus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Selenomonas ruminantium
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Vibrio vulnificus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine
-
 Selenomonas ruminantium cadaverine + CO2
-
 ?
L-lysine
-
 Vibrio vulnificus cadaverine + CO2
-
 ?
L-lysine 58% activity compared to L-ornithine Vibrio vulnificus cadaverine + CO2
-
 ?
L-ornithine
-
 Selenomonas ruminantium putrescine + CO2
-
 ?
L-ornithine
-
 Vibrio vulnificus putrescine + CO2
-
 ?
L-ornithine best substrate Vibrio vulnificus putrescine + CO2
-
 ?
additional information substrate specificity, overview. No activity with 2,3-diaminopropionic acid, 2,4-diaminobutric acid, and 2,7-diaminoheptanoic acid Vibrio vulnificus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44700, recombinant His6-tagged enzyme, SDS-PAGE Vibrio vulnificus
? x * 44200, recombinant His6-tagged enzyme, SDS-PAGE Selenomonas ruminantium

Synonyms

Synonyms Comment Organism
lysine/ornithine decarboxylase
-
 Selenomonas ruminantium
lysine/ornithine decarboxylase
-
 Vibrio vulnificus
More cf. EC 4.1.1.17 Vibrio vulnificus
SrLDC
-
 Selenomonas ruminantium
vvLDC
-
 Vibrio vulnificus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Selenomonas ruminantium
37
-
 assay at Vibrio vulnificus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme Selenomonas ruminantium
9
-
 recombinant enzyme Vibrio vulnificus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 enzyme vvLDC exhibits effective activity in alkaline pH conditions Vibrio vulnificus
8 10 50% of maximal activity at pH 8.0 and pH 10.0, maximal activity at pH 9.0, conversion rate at pH 9.0 is 40% Vibrio vulnificus
8 9 enzyme srLDC does not exhibit effective activity in alkaline pH conditions, the conversion rate of about 25% is similar at pH 8.0 and pH 9.0, no activity at pH 10.0 Selenomonas ruminantium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Selenomonas ruminantium
pyridoxal 5'-phosphate
-
 Vibrio vulnificus

General Information

General Information Comment Organism
additional information compared to the activity of lysine/ornithine decarboxylase from Selenomonas ruminantium and lysine decarboxylase from Geobacillus thermodenitrificans, the cadaverine producing activity of enzyme vvLDC is 1.5fold and 5.3fold increased, respectively Vibrio vulnificus
additional information compared to the lysine decarboxylase from Geobacillus thermodenitrificans, the cadaverine producing activity of enzyme srLDC is increased, but compared to the lysine/ornithine decarboxylase from Vibrio vulnificus, it is 1.5fold reduced Selenomonas ruminantium