Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 1 M (NH4)2SO4, 0.1 M Bis-Tris, pH 5.5, 1% (w/v) PEG 3350 or 0.1 M HEPES, pH 7.5, 0.2 M MgCl2, 10% (w/v) PEG 400 | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains a Mg2+ binding site | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57614 | - |
2 * 57614, SeMet-substituted protein, calculated from amino acid sequence | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose bead chromatography and Sephacryl S200 gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-octaprenyl-4-hydroxybenzoate | - |
Pseudomonas aeruginosa | 2-octaprenylphenol + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 57614, SeMet-substituted protein, calculated from amino acid sequence | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
PA0254 | - |
Pseudomonas aeruginosa |
ubiD | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not bind FMN | Pseudomonas aeruginosa |