Literature summary for 4.1.1.98 extracted from

Do, H.; Lee, C.W.; Han, S.J.; Lee, S.G.; Kim, H.J.; Park, H.; Lee, J.H.
Purification, crystallization and preliminary X-ray crystallographic studies of FMN-bound and FMN-free forms of aromatic acid decarboxylase (CpsUbiX) from the psychrophilic bacterium Colwellia psychrerythraea 34H (2014), Acta Crystallogr. Sect. F, 70, 215-220.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Colwellia psychrerythraea

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.1 M trisodium citrate pH 5.4, 0.5 M ammonium sulfate, 1.2 M lithium sulfate	Colwellia psychrerythraea

Protein Variants

Protein Variants	Comment	Organism
V47S	a FMN-free mutant	Colwellia psychrerythraea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22552	-	12 * 22552, calculated from amino acid sequence	Colwellia psychrerythraea
256300	-	gel filtration	Colwellia psychrerythraea

Organism

Organism	UniProt	Comment	Textmining
Colwellia psychrerythraea	Q489U8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration	Colwellia psychrerythraea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-octaprenyl-4-hydroxybenzoate	-	Colwellia psychrerythraea	2-octaprenylphenol + CO2	-	?

Subunits

Subunits	Comment	Organism
dodecamer	12 * 22552, calculated from amino acid sequence	Colwellia psychrerythraea

Synonyms

Synonyms	Comment	Organism
flavin mononucleotide-dependent aromatic acid decarboxylase	-	Colwellia psychrerythraea
ubiX	-	Colwellia psychrerythraea

Cofactor

Cofactor	Comment	Organism	Structure
FMN	dependent on	Colwellia psychrerythraea

pI Value

Organism	Comment	pI Value Maximum	pI Value
Colwellia psychrerythraea	calculated from amino acid sequence	-	5.8

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Release 2023.1 (January 2023)