Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Vibrio vulnificus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
carboxyspermidine | pH and temperature not specified in the publication | Vibrio vulnificus | |
0.27 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Vibrio vulnificus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nitrilotriacetic acid affinity resin column chromatography | Vibrio vulnificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxynorspermidine | carboxynorspermidine is the primary substrate and is decarboxylated 20fold more efficiently than carboxyspermidine | Vibrio vulnificus | bis(3-aminopropyl)amine + CO2 | - |
? | |
carboxyspermidine | - |
Vibrio vulnificus | spermidine + CO2 | - |
? | |
L-ornithine | the enzyme also exhibits some activity with L-ornithine | Vibrio vulnificus | ? | - |
? | |
additional information | the enzyme exhibits no activity with L-arginine, L-lysine, and meso-diaminopimelate | Vibrio vulnificus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 45000, SDS-PAGE | Vibrio vulnificus |
Synonyms | Comment | Organism |
---|---|---|
CANSDC | - |
Vibrio vulnificus |
carboxyspermidine decarboxylase | - |
Vibrio vulnificus |
nspC | - |
Vibrio vulnificus |
VC1623 | - |
Vibrio vulnificus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
carboxyspermidine | pH and temperature not specified in the publication | Vibrio vulnificus | |
20.1 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Vibrio vulnificus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Vibrio vulnificus |
General Information | Comment | Organism |
---|---|---|
metabolism | the CANSDH/CANSDC alternative polyamine biosynthetic pathway is able to synthesize both sym-norspermidine and spermidine | Vibrio vulnificus |
physiological function | the enzyme is essential for biofilm formation | Vibrio vulnificus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
carboxyspermidine | pH and temperature not specified in the publication | Vibrio vulnificus | |
74 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Vibrio vulnificus |