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Literature summary for 4.1.1.93 extracted from

  • Wieser, M.; Fujii, N.; Yoshida, T.; Nagasawa T.
    Carbon dioxide fixation by reversible pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910 (1998), Eur. J. Biochem., 257, 495-499.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) Priestia megaterium
ascorbate reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
Pyrrole substrate inhibition of carboxylation above 300 mM Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
61
-
Pyrrole pH 5.5, 20°C Priestia megaterium
560
-
HCO3- pH 5.5, 20°C Priestia megaterium

Metals/Ions

Metals/Ions Comment Organism Structure
Na2S2O5 reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-
Priestia megaterium PYR2910
-
-
-

Reaction

Reaction Comment Organism Reaction ID
pyrrole-2-carboxylate + H2O = pyrrole + HCO3- the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium
pyrrole-2-carboxylate = pyrrole + CO2 the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no carboxylation of thiophene, furan, indole, 1-methylpyrrole, 1-(2-cyanethyl)pyrrole, pyrrolidine, pyrazine, pyridine, pyridine-4-carboxamide, imidazole, n-butylimidazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,5-triazine and thiazole Priestia megaterium ?
-
?
additional information no carboxylation of thiophene, furan, indole, 1-methylpyrrole, 1-(2-cyanethyl)pyrrole, pyrrolidine, pyrazine, pyridine, pyridine-4-carboxamide, imidazole, n-butylimidazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,5-triazine and thiazole Priestia megaterium PYR2910 ?
-
?
pyrrole + HCO3- the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium pyrrole-2-carboxylate + H2O
-
?
pyrrole + HCO3- the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium PYR2910 pyrrole-2-carboxylate + H2O
-
?
pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium pyrrole + CO2
-
?
pyrrole-2-carboxylate the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide Priestia megaterium PYR2910 pyrrole + CO2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at, carboxylase reaction Priestia megaterium
30
-
assay at, decarboxylase reaction Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at, carboxylase reaction Priestia megaterium
7
-
assay at, decarboxylase reaction Priestia megaterium

General Information

General Information Comment Organism
physiological function despite a strong reverse reaction, it is assumed that reversible pyrrole-2-carboxylate decarboxylase takes part in the catabolism of pyrrole-2-carboxylate due to a slow decrease of the decarboxylation product pyrrole under resting cells conditions and the non-physiologically high Km-value for HCO23, which is substantially above the concentrations of HCO23 that probably occur in vivo Priestia megaterium