Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) | Priestia megaterium | |
ascorbate | reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pyrrole | substrate inhibition of carboxylation above 300 mM | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61 | - |
Pyrrole | pH 5.5, 20°C | Priestia megaterium | |
560 | - |
HCO3- | pH 5.5, 20°C | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na2S2O5 | reducing agents in optimal concentrations of 20 mM or above are a prerequisite for high CO2 fixation turnovers, with dithiothreitol enhancing the carboxylation 16.2fold compared with a control without reducing agent, followed by ascorbate (15.5fold), Na2S2O5 (13.6fold) and 2-mercaptoethanol (7.2fold) | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium PYR2910 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pyrrole-2-carboxylate + H2O = pyrrole + HCO3- | the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide | Priestia megaterium | |
pyrrole-2-carboxylate = pyrrole + CO2 | the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no carboxylation of thiophene, furan, indole, 1-methylpyrrole, 1-(2-cyanethyl)pyrrole, pyrrolidine, pyrazine, pyridine, pyridine-4-carboxamide, imidazole, n-butylimidazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,5-triazine and thiazole | Priestia megaterium | ? | - |
? | |
additional information | no carboxylation of thiophene, furan, indole, 1-methylpyrrole, 1-(2-cyanethyl)pyrrole, pyrrolidine, pyrazine, pyridine, pyridine-4-carboxamide, imidazole, n-butylimidazole, 1,2,3-triazole, 1,2,4-triazole, 1,3,5-triazine and thiazole | Priestia megaterium PYR2910 | ? | - |
? | |
pyrrole + HCO3- | the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide | Priestia megaterium | pyrrole-2-carboxylate + H2O | - |
? | |
pyrrole + HCO3- | the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide | Priestia megaterium PYR2910 | pyrrole-2-carboxylate + H2O | - |
? | |
pyrrole-2-carboxylate | the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide | Priestia megaterium | pyrrole + CO2 | - |
? | |
pyrrole-2-carboxylate | the enzyme catalyses both the carboxylation and decarboxylation reactions. However, while bicarbonate is the preferred substrate for the carboxylation reaction, decarboxylation produces carbon dioxide | Priestia megaterium PYR2910 | pyrrole + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at, carboxylase reaction | Priestia megaterium |
30 | - |
assay at, decarboxylase reaction | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at, carboxylase reaction | Priestia megaterium |
7 | - |
assay at, decarboxylase reaction | Priestia megaterium |
General Information | Comment | Organism |
---|---|---|
physiological function | despite a strong reverse reaction, it is assumed that reversible pyrrole-2-carboxylate decarboxylase takes part in the catabolism of pyrrole-2-carboxylate due to a slow decrease of the decarboxylation product pyrrole under resting cells conditions and the non-physiologically high Km-value for HCO23, which is substantially above the concentrations of HCO23 that probably occur in vivo | Priestia megaterium |