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Literature summary for 4.1.1.90 extracted from

  • Wu, S.M.; Morris, D.P.; Stafford, D.W.
    Identification and purification to near homogeneity of the vitamin K-dependent carboxylase (1991), Proc. Natl. Acad. Sci. USA, 88, 2236-2240.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
FFRCK peptide, protease inhibitor Bos taurus
FPRCK peptide, protease inhibitor Bos taurus
protease inhibitor mixture PIC, freshly prepared as a 10x PIC stock containing 20 mM dithiothreitol, 20 mM EDTA, FFRCK (1.25 microg/ml), FPRCK (1.25 microg/ml), leupeptin (5 microg/ml), pepstatin A (7 microg/ml), phenylmethylsulfonyl fluoride (340 microg/ml), aprotinin (20 microg/ml) Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus Q07175
-
-

Purification (Commentary)

Purification (Comment) Organism
degree of purification is about 7000fold with reference to ammonium sulfate-fractionated microsomal protein from liver. Purification of carboxylase, solubilized microsomes: 281000 cpm/mg/h, flow-through of Affi-FIXQ/S: 200000 cpm/mg/h, bound to Affi-FIXQ/S: 140000000 cpm/mg/h, affinity-purified carboxylase: 1930000000 cpm/mg/h Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FLEEL + CO2 + O2 + vitamin KH2 Phe-Leu-Glu-Glu-Leu Bos taurus ? + vitamin K epoxide + H2O
-
?
L-glutamate + CO2 + O2 + vitamin K hydroquinone catalyzes modification of specific glutamic acids to gamma-carboxyglutamic acid in several blood-coagulation proteins Bos taurus gamma-carboxy-L-glutamate + vitamin K epoxide + H2O
-
?
proFIXl9 + CO2 + O2 + vitamin KH2 AVFLDHENANKILNRPKRY Bos taurus ? + vitamin K epoxide + H2O
-
?

Subunits

Subunits Comment Organism
? x * 94000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
glutamate carboxylase
-
Bos taurus
vitamin K-dependent carboxylase
-
Bos taurus

Cofactor

Cofactor Comment Organism Structure
vitamin K
-
Bos taurus