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Literature summary for 4.1.1.90 extracted from

  • Czerwiec, E.; Begley, G.S.; Bronstein, M.; Stenflo, J.; Taylor, K.; Furie, B.C.; Furie, B.
    Expression and characterization of recombinant vitamin K-dependent gamma-glutamyl carboxylase from an invertebrate, Conus textile (2002), Eur. J. Biochem., 269, 6162-6172.
    View publication on PubMed
Search for more literature for 4.1.1.90

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS cells or expressed in Sf21 insect cells Conus textile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 28-residue peptides based on residues -18 to +10 of human proprothrombin pH 7.0 Conus textile
0.006
-
 proFactor IX pH 7.0 Conus textile
0.052
-
 reduced vitamin K1 pH 7.0 Conus textile
0.074
-
 precursor analog containing 29 amino acids of the propeptide region pH 7.0 Conus textile
0.075
-
 precursor analog containing 12 of the propeptide region pH 7.0 Conus textile
0.42
-
 FLEEL pH 7.0 Conus textile
0.565
-
 conotoxin epsilon-TxIX pH 7.0 Conus textile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 cells transfected with the CAT-V5/His plasmid, Western Blot Conus textile
130000
-
 SDS-PAGE, Sf21 cells transfected with the carboxylase cDNA-containing plasmid, Western Blot Conus textile

Organism

Organism UniProt Comment Textmining
Conus textile Q8IA33
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
28-residue peptides based on residues -18 to +10 of human proprothrombin + CO2 + O2 + reduced vitamin K1
-
 Conus textile ?
-
 ?
conotoxin epsilon-TxIX + CO2 + O2 + vitamin KH2
-
 Conus textile ?
-
 ?
e-TxIX12 + CO2 + O2 + vitamin KH2 residues 1-12 of e-TxIX Conus textile ?
-
 ?
FLEEL + CO2 + O2 + reduced vitamin K1
-
 Conus textile ?
-
 ?
FLEEL + CO2 + O2 + vitamin KH2
-
 Conus textile ? + vitamin K epoxide + H2O
-
 ?
L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
 Conus textile gamma-carboxy L-glutamate + vitamin K epoxide + H2O
-
 ?
additional information amino-acid sequences of the synthetic substrates and propeptides are shown Conus textile ?
-
 ?
precursor analog containing 12 of the propeptide region + CO2 + O2 + reduced vitamin K1
-
 Conus textile ?
-
 ?
precursor analog containing 29 amino acids of the propeptide region + CO2 + O2 + reduced vitamin K1
-
 Conus textile ?
-
 ?
pro-e-TxIX/12 + CO2 + O2 + vitamin KH2 residues -12 to -1 of e-TxIX precursor Conus textile ?
-
 ?
pro-e-TxIX/24 + CO2 + O2 + vitamin KH2 residues -12 to +12 of e-TxIX precursor Conus textile ?
-
 ?
pro-e-TxIX/41 + CO2 + O2 + vitamin KH2 residues -29 to +12 of e-TxIX precursor Conus textile ?
-
 ?
proFactor IX + CO2 + O2 + reduced vitamin K1
-
 Conus textile ?
-
 ?
proFIX18 + CO2 + O2 + vitamin KH2 residues -18 to -1 of proFactor IX Conus textile ?
-
 ?
proFIX28 + CO2 + O2 + vitamin KH2 residues -18 to +10 of proFactor IX Conus textile ?
-
 ?
proPT18 + CO2 + O2 + vitamin KH2 residues -18 to -1 of proprothrombin. 28-residue peptides based on residues -18 to +10 of human proprothrombin and proFactor IX with Km values of 420 lM, 1.7 microM and 6 microM Conus textile ?
-
 ?
proPT28 + CO2 + O2 + vitamin KH2 residues -18 to +10 of proprothrombin Conus textile ?
-
 ?

Synonyms

Synonyms Comment Organism
gamma-glutamyl carboxylase
-
 Conus textile
glutamate carboxylase
-
 Conus textile

Cofactor

Cofactor Comment Organism Structure
vitamin K1
-
 Conus textile