Literature summary for 4.1.1.90 extracted from

Yamada, M.; Kuliopulos, A.; Nelson, N.P.; Roth, D.A.; Furie, B.; Furie, B.C.; Walsh, C.T.
Localization of the factor IX propeptide binding site on recombinant vitamin K dependent carboxylase using benzoylphenylalanine photoaffinity peptide inactivators: carboxylase using benzoylphenylalanine photoaffinity peptide inactivators (1995), Biochemistry, 34, 481-489.

Search for more literature for 4.1.1.90

Activating Compound

Activating Compound	Comment	Organism	Structure
benzoylphenylalanine	Bpa, the four Bpa peptides enhance gamma-carboxylation by 1.5-2.3fold, and the rate enhancement profiles are very similar to that of proFIX19, showing that these propeptides are recognized by the carboxylase	Bos taurus
proFIX19	enhances gamma-carboxylation of the synthetic FLEEL peptide by 2.2-2.3fold	Bos taurus
proPT18	enhances gamma-carboxylation of the synthetic FLEEL peptide by 2.2-2.3fold	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
His6-tagged bovine liver carboxylase (His6-carboxylase) is produced in insect cells using a baculovirus expression system	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
proFIX19-16BPA propeptide	-	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km-values measured by hyperbolic weighted least-squares analysis	Bos taurus
1.5	-	FLEEL	proPTl8 propeptide	Bos taurus
1.6	-	FLEEL	proFIX19 propeptide	Bos taurus
1.8	-	FLEEL	proFIX 19-6BPA propeptide	Bos taurus
1.9	-	FLEEL	proFIX19-16BPA propeptide	Bos taurus
2	-	FLEEL	proFIX19-13BPA propeptide	Bos taurus
2	-	FLEEL	proFIX19-7BPApropeptide	Bos taurus
9	-	FLEEL	none propeptide	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Bos taurus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
94000	-	SDS-PAGE	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	Q07175	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
FLEEL + CO2 + O2 + vitamin KH2	-	Bos taurus	? + vitamin K epoxide + H2O	-	?
additional information	vitamin K carboxylase specifically interacts with the propeptide region of the precursor forms of vitamin K dependent proteins	Bos taurus	?	-	?
N-(bromoacetyl)-FLEELY + CO2 + O2 + vitamin KH2	-	Bos taurus	? + vitamin K epoxide + H2O	-	?
ProFIX 19-6BPA + CO2 + O2 + FLEEL + vitamin KH2	TVFLDHENANKIBNRPKR	Bos taurus	? + vitamin K epoxide + H2O	-	?
ProFIX 19-7BPA + CO2 + O2 + FLEEL + vitamin KH2	TVFLDHENfiNKBLNRPKR	Bos taurus	? + vitamin K epoxide + H2O	-	?
proFIX19 + CO2 + O2 + FLEEL + vitamin KH2	TVFLDHENANKILNRPKRY	Bos taurus	? + vitamin K epoxide + H2O	-	?
ProFIX19-13BPA + CO2 + O2 + FLEEL + vitamin KH2	TVFLDBENWKILNRPKRY	Bos taurus	? + vitamin K epoxide + H2O	-	?
ProFIX19-16BPA + CO2 + O2 + FLEEL + vitamin KH2	TVBLDHENANKILNRPKRY	Bos taurus	? + vitamin K epoxide + H2O	-	?
proPT28 + CO2 + O2 + FLEEL + vitamin KH2	HVFLAPQQARSLLQRVRRANTFLEEVRK	Bos taurus	? + vitamin K epoxide + H2O	-	?
proPTl8 + CO2 + O2 + FLEEL + vitamin KH2	HVFLAPQQARSLLQRVRR	Bos taurus	? + vitamin K epoxide + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 94000, SDS-PAGE	Bos taurus

Synonyms

Synonyms	Comment	Organism
glutamate carboxylase	-	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
vitamin K	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0174	-	proFIX19-16BPA propeptide	competitive inhibition experiments using prom28 substrate	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)