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Literature summary for 4.1.1.9 extracted from

  • Dyck, J.R.B.; Berthiaume, L.G.; Thomas, P.D.; Kantor, P.F.; Barr, A.J.; Barr, R.; Singh, D.; Hopkins, T.A.; Voilley, N.; Prentki, M.; Lopaschuk, G.D.
    Characterization of rat liver malonyl-CoA decarboxylase and the study of its role in regulating fatty acid metabolism (2000), Biochem. J., 350, 599-608.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Alkaline phosphatase treatment with alkaline phosphatase enhances MCD activity suggesting phosphorylation-related control of enzyme Rattus norvegicus
additional information MCD activity is elevated under conditions in which hepatic fatty acid oxidation is known to increase, such as streptozotocin-induced diabetes or following a 48 h fast, 2fold increase in expression in streptozotocin-diabetic rats compared with controls Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
from liver, expression in COS-7 cells as 50.7 kDa and 54.7 kDa MCD isoforms, sequencing Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50700
-
x * 50700, liver isoform, MCD exists in 2 isoforms of 50.7 kDa and 54.7 kDa, SDS-PAGE, Western blot analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Malonyl-CoA Rattus norvegicus important role in regulating fatty acid metabolism, involved in the control of cellular malonyl-CoA levels, may play an essential role in regulating energy utilization in the liver by regulating malonyl-CoA levels in response to various nutritional or pathological states, MCD regulation from liver Acetyl-CoA + CO2
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male sprague-dawley rats
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the 50.7 kDa isoform may be generated from the 54.7 kDa isoform by posttranslational processing, probably by N-terminal cleavage Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
50.7 kDa isoform from liver, 1269fold Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Malonyl-CoA
-
Rattus norvegicus Acetyl-CoA + CO2
-
?
Malonyl-CoA important role in regulating fatty acid metabolism, involved in the control of cellular malonyl-CoA levels, may play an essential role in regulating energy utilization in the liver by regulating malonyl-CoA levels in response to various nutritional or pathological states, MCD regulation from liver Rattus norvegicus Acetyl-CoA + CO2
-
?

Subunits

Subunits Comment Organism
? x * 50700, liver isoform, MCD exists in 2 isoforms of 50.7 kDa and 54.7 kDa, SDS-PAGE, Western blot analysis Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rattus norvegicus