Literature summary for 4.1.1.9 extracted from
Dyck, J.R.B.; Berthiaume, L.G.; Thomas, P.D.; Kantor, P.F.; Barr, A.J.; Barr, R.; Singh, D.; Hopkins, T.A.; Voilley, N.; Prentki, M.; Lopaschuk, G.D.
Characterization of rat liver malonyl-CoA decarboxylase and the study of its role in regulating fatty acid metabolism (2000), Biochem. J., 350, 599-608.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
Alkaline phosphatase |
treatment with alkaline phosphatase enhances MCD activity suggesting phosphorylation-related control of enzyme |
Rattus norvegicus |
|
additional information |
MCD activity is elevated under conditions in which hepatic fatty acid oxidation is known to increase, such as streptozotocin-induced diabetes or following a 48 h fast, 2fold increase in expression in streptozotocin-diabetic rats compared with controls |
Rattus norvegicus |
|
Cloned(Commentary)
Cloned (Comment) |
Organism |
from liver, expression in COS-7 cells as 50.7 kDa and 54.7 kDa MCD isoforms, sequencing |
Rattus norvegicus |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
50700 |
- |
x * 50700, liver isoform, MCD exists in 2 isoforms of 50.7 kDa and 54.7 kDa, SDS-PAGE, Western blot analysis |
Rattus norvegicus |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
Malonyl-CoA |
Rattus norvegicus |
important role in regulating fatty acid metabolism, involved in the control of cellular malonyl-CoA levels, may play an essential role in regulating energy utilization in the liver by regulating malonyl-CoA levels in response to various nutritional or pathological states, MCD regulation from liver |
Acetyl-CoA + CO2 |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Rattus norvegicus |
- |
male sprague-dawley rats |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
proteolytic modification |
the 50.7 kDa isoform may be generated from the 54.7 kDa isoform by posttranslational processing, probably by N-terminal cleavage |
Rattus norvegicus |
Purification (Commentary)
Purification (Comment) |
Organism |
50.7 kDa isoform from liver, 1269fold |
Rattus norvegicus |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
additional information |
- |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
Malonyl-CoA |
- |
Rattus norvegicus |
Acetyl-CoA + CO2 |
- |
? |
|
Malonyl-CoA |
important role in regulating fatty acid metabolism, involved in the control of cellular malonyl-CoA levels, may play an essential role in regulating energy utilization in the liver by regulating malonyl-CoA levels in response to various nutritional or pathological states, MCD regulation from liver |
Rattus norvegicus |
Acetyl-CoA + CO2 |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
? |
x * 50700, liver isoform, MCD exists in 2 isoforms of 50.7 kDa and 54.7 kDa, SDS-PAGE, Western blot analysis |
Rattus norvegicus |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
37 |
- |
assay at |
Rattus norvegicus |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
8 |
- |
assay at |
Rattus norvegicus |