Literature summary for 4.1.1.88 extracted from

Maderbocus, R.; Fields, B.L.; Hamilton, K.; Luo, S.; Tran, T.H.; Dietrich, L.E.P.; Tong, L.
Crystal structure of a Pseudomonas malonate decarboxylase holoenzyme hetero-tetramer (2017), Nat. Commun., 8, 160 .

Search for more literature for 4.1.1.88

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	the enzyme is independent from biotin	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21Star (DE3), BL21 Rosetta, and B834 (DE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
MdcD-MdcE subunits, sitting drop vapor diffusion method, using 0.1 M Bis-Tris (pH 5.8), 18% (w/v) PEG 3350, and 0.2 M ammonium acetate. Holoenzyme in complex with CoA and malonate, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 8% (w/v) tacsimate	Pseudomonas aeruginosa
the holoenzyme contains four subunits, having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities. Structure of the MdcD-MdcE complex at 1.86 A resolution, and overall strcuture of the heterotetramer at 2.2 A resolution	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
Q100E	the mutant of subunit MdcE has a roughly 10fold lower catalytic activity compared to the wild type enzyme	Pseudomonas aeruginosa
Q100E/Y102F	inactive	Pseudomonas aeruginosa
S142A	the mutant of subunit MdcE has a roughly 10fold lower catalytic activity compared to the wild type enzyme	Pseudomonas aeruginosa
Y102F	the mutant of subunit MdcE has about wild type catalytic activity	Pseudomonas aeruginosa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Pseudomonas aeruginosa	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
140000	-	gel filtration	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
malonate + H+	Pseudomonas aeruginosa	-	acetate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I6S7 and Q9I6S6	beta subunit MdcD and gamma subunit MdcE	-
Pseudomonas aeruginosa	Q9I6T0 and Q9I6S6	Q9I6T0 i.e. subunit MdcA, Q9I6S6 i.e. subunit MdcE	-
Pseudomonas aeruginosa DSM 22644	Q9I6T0 and Q9I6S6	Q9I6T0 i.e. subunit MdcA, Q9I6S6 i.e. subunit MdcE	-

Purification (Commentary)

Purification (Comment)	Organism
nickel bead column chromatography and Sephacryl S-300 gel filtration	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
malonate + H+	-	Pseudomonas aeruginosa	acetate + CO2	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	x-ray crystallography	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
biotin-independent malonate decarboxylase	the holoenzyme contains four subunits having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities	Pseudomonas aeruginosa
MdcACDE	-	Pseudomonas aeruginosa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)