BRENDA - Enzyme Database
show all sequences of 4.1.1.88

Purification and properties of a novel type of malonate decarboxylase from Acinetobacter calcoaceticus

Kim, Y.S.; Byun, H.S.; J. Biol. Chem. 269, 29636-29641 (1994)

Data extracted from this reference:

General Stability
General Stability
Organism
enzyme is sensitive to repeated freezing and thawing
Acinetobacter calcoaceticus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Nitro-5-thiocyanobenzoic acid
0.64 mM, 2.1% residual activity
Acinetobacter calcoaceticus
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 14.5% residual activity
Acinetobacter calcoaceticus
iodoacetamide
1 mM, 58% residual activity
Acinetobacter calcoaceticus
N-ethylmaleimide
1 mM, 1% residual activity
Acinetobacter calcoaceticus
Sodium borohydride
inactivation
Acinetobacter calcoaceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
malonate
pH 6.8, 30°C
Acinetobacter calcoaceticus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
180000
-
PAGE
Acinetobacter calcoaceticus
185000
-
gel filtration
Acinetobacter calcoaceticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
growth on malonate
-
Purification (Commentary)
Commentary
Organism
-
Acinetobacter calcoaceticus
Reaction
Reaction
Commentary
Organism
malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2]
malonate decarboxylation proceeds by a catalytic cycle in which the acetyl group on the active enzyme is displaced by malonate, which binds covalently to a thiol group on the enzyme and is subsequently decarboxylated
Acinetobacter calcoaceticus
Renatured (Commentary)
Commentary
Organism
reactivation of deacetylated enzyme by incubation with acetic anhydride or malonyl-CoA
Acinetobacter calcoaceticus
Storage Stability
Storage Stability
Organism
-20°C, stable for several months
Acinetobacter calcoaceticus
4°C, deacetylated enzyme, full reactivation by addition of malonyl-CoA during 2 months storage
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ethylmalonate + H+
0.8% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
2-methylmalonate + H+
24% of the activity with malonate
687489
Acinetobacter calcoaceticus
propionate + CO2
-
-
-
?
glutarate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
malonate + H+
enzyme is highly specific for malonate
687489
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
oxalate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Acinetobacter calcoaceticus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the alpha subunit contains a biotin prosthetic group which does not seem to be directly related to malonate decarboxylation
Acinetobacter calcoaceticus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Acinetobacter calcoaceticus
isoelectric focusing
-
6.4
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the alpha subunit contains a biotin prosthetic group which does not seem to be directly related to malonate decarboxylation
Acinetobacter calcoaceticus
General Stability (protein specific)
General Stability
Organism
enzyme is sensitive to repeated freezing and thawing
Acinetobacter calcoaceticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Nitro-5-thiocyanobenzoic acid
0.64 mM, 2.1% residual activity
Acinetobacter calcoaceticus
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 14.5% residual activity
Acinetobacter calcoaceticus
iodoacetamide
1 mM, 58% residual activity
Acinetobacter calcoaceticus
N-ethylmaleimide
1 mM, 1% residual activity
Acinetobacter calcoaceticus
Sodium borohydride
inactivation
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
malonate
pH 6.8, 30°C
Acinetobacter calcoaceticus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Acinetobacter calcoaceticus
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
180000
-
PAGE
Acinetobacter calcoaceticus
185000
-
gel filtration
Acinetobacter calcoaceticus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Acinetobacter calcoaceticus
Renatured (Commentary) (protein specific)
Commentary
Organism
reactivation of deacetylated enzyme by incubation with acetic anhydride or malonyl-CoA
Acinetobacter calcoaceticus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for several months
Acinetobacter calcoaceticus
4°C, deacetylated enzyme, full reactivation by addition of malonyl-CoA during 2 months storage
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-ethylmalonate + H+
0.8% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
2-methylmalonate + H+
24% of the activity with malonate
687489
Acinetobacter calcoaceticus
propionate + CO2
-
-
-
?
glutarate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
butanoate + CO2
-
-
-
?
malonate + H+
enzyme is highly specific for malonate
687489
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
oxalate + H+
1.1% of the activity with malonate
687489
Acinetobacter calcoaceticus
CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit
Acinetobacter calcoaceticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Acinetobacter calcoaceticus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Acinetobacter calcoaceticus
isoelectric focusing
-
6.4
Other publictions for EC 4.1.1.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748747
Maderbocus
Crystal structure of a Pseudo ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644
Nat. Commun.
8
160
2017
1
-
1
1
4
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1
-
1
1
-
6
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1
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1
1
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1
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1
-
2
4
-
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1
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1
1
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1
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-
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1
1
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-
-
-
-
-
-
-
-
-
-
-
-
685057
Hoenke
Identification of the active s ...
Klebsiella pneumoniae
Biochemistry
39
13233-13240
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
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-
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1
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-
-
-
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-
-
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-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684660
Handa
Stereochemical course of bioti ...
Acinetobacter calcoaceticus, Pseudomonas fluorescens
Arch. Biochem. Biophys.
370
93-96
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
5
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2
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-
-
-
-
-
686494
Koo
Functional evaluation of the g ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
266
683-690
1999
-
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
1
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2
1
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1
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1
1
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-
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3
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-
-
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-
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1
-
2
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
685309
Koo
Cloning and sequencing of gene ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1354
49-54
1997
-
-
1
-
1
-
-
-
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5
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1
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1
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1
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686492
Hoenke
Sequence of a gene cluster fro ...
Klebsiella pneumoniae
Eur. J. Biochem.
246
530-538
1997
-
-
1
-
-
-
-
-
-
-
-
-
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4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687464
Byun
-
Subunit organization of bacter ...
Acinetobacter calcoaceticus, Pseudomonas fluorescens
J. Biochem. Mol. Biol.
30
132-137
1997
3
-
-
-
-
-
12
-
-
-
10
-
-
2
-
2
2
-
2
-
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
12
-
-
-
-
10
-
-
-
2
2
2
-
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686490
Schmid
Malonate decarboxylase of Kleb ...
Klebsiella pneumoniae
Eur. J. Biochem.
237
221-228
1996
-
-
-
-
-
-
2
-
-
-
4
-
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2
-
-
1
1
1
-
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-
-
2
-
-
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-
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2
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4
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1
1
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2
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-
-
-
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-
-
-
-
-
-
-
687489
Kim
Purification and properties of ...
Acinetobacter calcoaceticus
J. Biol. Chem.
269
29636-29641
1994
-
-
-
-
-
1
5
1
1
-
5
-
-
2
-
-
1
1
1
-
-
2
5
1
-
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-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
1
-
5
-
1
1
-
5
-
-
-
-
1
1
-
-
2
5
1
-
-
-
-
1
-
-
1
-
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-
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