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Literature summary for 4.1.1.88 extracted from

  • Kim, Y.S.; Byun, H.S.
    Purification and properties of a novel type of malonate decarboxylase from Acinetobacter calcoaceticus (1994), J. Biol. Chem., 269, 29636-29641.
    View publication on PubMed
Search for more literature for 4.1.1.88

General Stability

General Stability Organism
enzyme is sensitive to repeated freezing and thawing Acinetobacter calcoaceticus

Inhibitors

Inhibitors Comment Organism Structure
2-Nitro-5-thiocyanobenzoic acid 0.64 mM, 2.1% residual activity Acinetobacter calcoaceticus
5,5'-dithio-bis(2-nitrobenzoic acid) 0.5 mM, 14.5% residual activity Acinetobacter calcoaceticus
iodoacetamide 1 mM, 58% residual activity Acinetobacter calcoaceticus
N-ethylmaleimide 1 mM, 1% residual activity Acinetobacter calcoaceticus
Sodium borohydride inactivation Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 malonate pH 6.8, 30°C Acinetobacter calcoaceticus

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Acinetobacter calcoaceticus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus
32000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus
65000
-
 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus
180000
-
 PAGE Acinetobacter calcoaceticus
185000
-
 gel filtration Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
 growth on malonate
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter calcoaceticus

Reaction

Reaction Comment Organism Reaction ID
malonate + H+ + Na+[side 1] = acetate + CO2 + Na+[side 2] malonate decarboxylation proceeds by a catalytic cycle in which the acetyl group on the active enzyme is displaced by malonate, which binds covalently to a thiol group on the enzyme and is subsequently decarboxylated Acinetobacter calcoaceticus
a

Renatured (Commentary)

Renatured (Comment) Organism
reactivation of deacetylated enzyme by incubation with acetic anhydride or malonyl-CoA Acinetobacter calcoaceticus

Storage Stability

Storage Stability Organism
-20°C, stable for several months Acinetobacter calcoaceticus
4°C, deacetylated enzyme, full reactivation by addition of malonyl-CoA during 2 months storage Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ethylmalonate + H+ 0.8% of the activity with malonate Acinetobacter calcoaceticus butanoate + CO2
-
 ?
2-methylmalonate + H+ 24% of the activity with malonate Acinetobacter calcoaceticus propionate + CO2
-
 ?
glutarate + H+ 1.1% of the activity with malonate Acinetobacter calcoaceticus butanoate + CO2
-
 ?
malonate + H+ enzyme is highly specific for malonate Acinetobacter calcoaceticus acetate + CO2
-
 ?
oxalate + H+ 1.1% of the activity with malonate Acinetobacter calcoaceticus CO2
-
 ?

Subunits

Subunits Comment Organism
multimer 2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
additional information the alpha subunit contains a biotin prosthetic group which does not seem to be directly related to malonate decarboxylation Acinetobacter calcoaceticus

pI Value

Organism Comment pI Value Maximum pI Value
Acinetobacter calcoaceticus isoelectric focusing
-
 6.4