BRENDA - Enzyme Database
show all sequences of 4.1.1.88

Subunit organization of bacterial malonate decarboxylases: The smallest delta subunit as an acyl-carrier protein

Byun, H.S.; Kim, Y.S.; J. Biochem. Mol. Biol. 30, 132-137 (1997)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
diethyldicarbonate
1 mM, 114% of initial activity
Pseudomonas fluorescens
N-Acetylimidazole
1 mM, 117% of initial activity
Pseudomonas fluorescens
pyridoxal 5'phosphate
1 mM, 112% of initial activity
Pseudomonas fluorescens
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 1.5% residual activity
Acinetobacter calcoaceticus
Bromoacetate
0.5 mM, 0.9% residual activity
Acinetobacter calcoaceticus
Bromoacetate
0.5 mM, 0.1% residual activity; 0.5 mM, 0.4% residual activity
Pseudomonas fluorescens
diethyldicarbonate
1 mM, 91% residual activity
Acinetobacter calcoaceticus
diethyldicarbonate
1 mM, 88% residual activity
Pseudomonas fluorescens
iodoacetamide
0.5 mM, 58% residual activity
Acinetobacter calcoaceticus
N-Acetylimidazole
1 mM, 95% residual activity
Acinetobacter calcoaceticus
N-Acetylimidazole
1 mM, 95% residual activity
Pseudomonas fluorescens
N-ethylmaleimide
0.5 mM, 0.1% residual activity
Acinetobacter calcoaceticus
N-ethylmaleimide
0.5 mM, 0.2% residual activity
Pseudomonas fluorescens
pyridoxal 5'phosphate
1 mM, 82% residual activity
Acinetobacter calcoaceticus
pyridoxal 5'phosphate
1 mM, 92% residual activity
Pseudomonas fluorescens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
11000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
30000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
33000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
65000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
185000
-
gel filtration
Acinetobacter calcoaceticus
185000
-
gel filtration
Pseudomonas fluorescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
grown on malonate
-
Pseudomonas fluorescens
-
grown on malonate
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Acinetobacter calcoaceticus
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Pseudomonas fluorescens
Purification (Commentary)
Commentary
Organism
-
Pseudomonas fluorescens
-
Acinetobacter calcoaceticus
Renatured (Commentary)
Commentary
Organism
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity
Acinetobacter calcoaceticus
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity; reactivation of deacetyl malonate decarboxylase with malonyl-CoA to 100%, with acetyl-CoA or methylmalonyl-CoA to about 50% of initial activity, respectively
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.48
-
30°C
Pseudomonas fluorescens
10.02
-
30°C
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + H+
-
687464
Pseudomonas fluorescens
acetate + CO2
-
-
-
?
malonate + H+
-
687464
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
multimer
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
diethyldicarbonate
1 mM, 114% of initial activity
Pseudomonas fluorescens
N-Acetylimidazole
1 mM, 117% of initial activity
Pseudomonas fluorescens
pyridoxal 5'phosphate
1 mM, 112% of initial activity
Pseudomonas fluorescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithio-bis(2-nitrobenzoic acid)
0.5 mM, 1.5% residual activity
Acinetobacter calcoaceticus
Bromoacetate
0.5 mM, 0.9% residual activity
Acinetobacter calcoaceticus
Bromoacetate
0.5 mM, 0.1% residual activity; 0.5 mM, 0.4% residual activity
Pseudomonas fluorescens
diethyldicarbonate
1 mM, 91% residual activity
Acinetobacter calcoaceticus
diethyldicarbonate
1 mM, 88% residual activity
Pseudomonas fluorescens
iodoacetamide
0.5 mM, 58% residual activity
Acinetobacter calcoaceticus
N-Acetylimidazole
1 mM, 95% residual activity
Acinetobacter calcoaceticus
N-Acetylimidazole
1 mM, 95% residual activity
Pseudomonas fluorescens
N-ethylmaleimide
0.5 mM, 0.1% residual activity
Acinetobacter calcoaceticus
N-ethylmaleimide
0.5 mM, 0.2% residual activity
Pseudomonas fluorescens
pyridoxal 5'phosphate
1 mM, 82% residual activity
Acinetobacter calcoaceticus
pyridoxal 5'phosphate
1 mM, 92% residual activity
Pseudomonas fluorescens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
11000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
25000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
30000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
32000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
33000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
65000
-
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
65000
-
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
185000
-
gel filtration
Acinetobacter calcoaceticus
185000
-
gel filtration
Pseudomonas fluorescens
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Acinetobacter calcoaceticus
additional information
the delta subunit of the active form of enzyme is acetylated, the acetyl group may form a thioester bond with the thiol group of the prosthetic group
Pseudomonas fluorescens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas fluorescens
-
Acinetobacter calcoaceticus
Renatured (Commentary) (protein specific)
Commentary
Organism
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity
Acinetobacter calcoaceticus
reactivation of deacetyl malonate decarboxylase with malonyl-CoA, acetyl-CoA or methylmalonyl-CoA to more than 80% of initial activity; reactivation of deacetyl malonate decarboxylase with malonyl-CoA to 100%, with acetyl-CoA or methylmalonyl-CoA to about 50% of initial activity, respectively
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.48
-
30°C
Pseudomonas fluorescens
10.02
-
30°C
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + H+
-
687464
Pseudomonas fluorescens
acetate + CO2
-
-
-
?
malonate + H+
-
687464
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
multimer
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit
Acinetobacter calcoaceticus
multimer
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit
Pseudomonas fluorescens
Other publictions for EC 4.1.1.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748747
Maderbocus
Crystal structure of a Pseudo ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644
Nat. Commun.
8
160
2017
1
-
1
1
4
-
-
-
1
-
1
1
-
6
-
-
1
-
-
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-
-
1
1
-
-
-
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-
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1
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1
-
2
4
-
-
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-
-
1
-
1
1
-
-
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1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685057
Hoenke
Identification of the active s ...
Klebsiella pneumoniae
Biochemistry
39
13233-13240
2000
-
-
-
-
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-
-
-
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4
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1
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1
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-
684660
Handa
Stereochemical course of bioti ...
Acinetobacter calcoaceticus, Pseudomonas fluorescens
Arch. Biochem. Biophys.
370
93-96
1999
-
-
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5
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2
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686494
Koo
Functional evaluation of the g ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
266
683-690
1999
-
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
1
-
-
-
-
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-
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1
-
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-
-
1
1
-
-
-
-
-
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3
-
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-
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1
-
2
1
-
-
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-
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-
-
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-
-
-
-
-
685309
Koo
Cloning and sequencing of gene ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1354
49-54
1997
-
-
1
-
1
-
-
-
-
-
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-
-
5
-
-
-
-
-
-
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1
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1
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1
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1
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-
-
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-
-
-
-
686492
Hoenke
Sequence of a gene cluster fro ...
Klebsiella pneumoniae
Eur. J. Biochem.
246
530-538
1997
-
-
1
-
-
-
-
-
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4
-
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1
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1
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1
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687464
Byun
-
Subunit organization of bacter ...
Acinetobacter calcoaceticus, Pseudomonas fluorescens
J. Biochem. Mol. Biol.
30
132-137
1997
3
-
-
-
-
-
12
-
-
-
10
-
-
2
-
2
2
-
2
-
2
-
2
2
-
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3
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12
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10
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2
2
2
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2
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2
2
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686490
Schmid
Malonate decarboxylase of Kleb ...
Klebsiella pneumoniae
Eur. J. Biochem.
237
221-228
1996
-
-
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-
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2
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4
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2
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1
1
1
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2
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2
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1
1
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2
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687489
Kim
Purification and properties of ...
Acinetobacter calcoaceticus
J. Biol. Chem.
269
29636-29641
1994
-
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1
5
1
1
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5
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2
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1
1
1
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2
5
1
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1
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1
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1
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1
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1
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5
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1
1
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5
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1
1
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2
5
1
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1
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1
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