BRENDA - Enzyme Database
show all sequences of 4.1.1.88

Functional evaluation of the genes involved in malonate decarboxylation by Acinetobacter calcoaceticus

Koo, J.H.; Kim, Y.S.; Eur. J. Biochem. 266, 683-690 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Acinetobacter calcoaceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
0.16
-
malonate
subunit beta in presence of acetyl-CoA; subunits beta/gamma in presence of acetyl-CoA
Acinetobacter calcoaceticus
3.23
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
the alpha subunit shows CoA-transferase activity with specific activity of 39.03 U/mg
Acinetobacter calcoaceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + H+
-
686494
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
malonate + H+
-
686494
Acinetobacter calcoaceticus MdcH
acetate + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein
Acinetobacter calcoaceticus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the prosthetic group of the acyl carrier protein is carboxymethylated 2'-(5''-phosphoribosyl)-3'-dephospho-CoA
Acinetobacter calcoaceticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Acinetobacter calcoaceticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the prosthetic group of the acyl carrier protein is carboxymethylated 2'-(5''-phosphoribosyl)-3'-dephospho-CoA
Acinetobacter calcoaceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
0.16
-
malonate
subunit beta in presence of acetyl-CoA; subunits beta/gamma in presence of acetyl-CoA
Acinetobacter calcoaceticus
3.23
-
malonate
apo-enzyme in presence of acetyl-CoA
Acinetobacter calcoaceticus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
the alpha subunit shows CoA-transferase activity with specific activity of 39.03 U/mg
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + H+
-
686494
Acinetobacter calcoaceticus
acetate + CO2
-
-
-
?
malonate + H+
-
686494
Acinetobacter calcoaceticus MdcH
acetate + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein
Acinetobacter calcoaceticus
Other publictions for EC 4.1.1.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748747
Maderbocus
Crystal structure of a Pseudo ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa DSM 22644
Nat. Commun.
8
160
2017
1
-
1
1
4
-
-
-
1
-
1
1
-
6
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
2
4
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685057
Hoenke
Identification of the active s ...
Klebsiella pneumoniae
Biochemistry
39
13233-13240
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684660
Handa
Stereochemical course of bioti ...
Acinetobacter calcoaceticus, Pseudomonas fluorescens
Arch. Biochem. Biophys.
370
93-96
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686494
Koo
Functional evaluation of the g ...
Acinetobacter calcoaceticus
Eur. J. Biochem.
266
683-690
1999
-
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685309
Koo
Cloning and sequencing of gene ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1354
49-54
1997
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686492
Hoenke
Sequence of a gene cluster fro ...
Klebsiella pneumoniae
Eur. J. Biochem.
246
530-538
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687464
Byun
-
Subunit organization of bacter ...
Acinetobacter calcoaceticus, Pseudomonas fluorescens
J. Biochem. Mol. Biol.
30
132-137
1997
3
-
-
-
-
-
12
-
-
-
10
-
-
2
-
2
2
-
2
-
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
12
-
-
-
-
10
-
-
-
2
2
2
-
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686490
Schmid
Malonate decarboxylase of Kleb ...
Klebsiella pneumoniae
Eur. J. Biochem.
237
221-228
1996
-
-
-
-
-
-
2
-
-
-
4
-
-
2
-
-
1
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687489
Kim
Purification and properties of ...
Acinetobacter calcoaceticus
J. Biol. Chem.
269
29636-29641
1994
-
-
-
-
-
1
5
1
1
-
5
-
-
2
-
-
1
1
1
-
-
2
5
1
-
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
1
-
5
-
1
1
-
5
-
-
-
-
1
1
-
-
2
5
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-