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Literature summary for 4.1.1.86 extracted from

  • Lee, J.; Sperandio, V.; Frantz, D.E.; Longgood, J.; Camilli, A.; Phillips, M.A.; Michael, A.J.
    An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae (2009), J. Biol. Chem., 284, 9899-9907.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.86

Cloned(Commentary)

Cloned (Comment) Organism
fusion protein DABA AT/DC cloned into pET28 vector, expressed in Escherichia coli BL21(DE3) Vibrio cholerae serotype O1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 L-2,4-diaminobutyrate DABA AT/DC fusion protein Vibrio cholerae serotype O1
11.1
-
 L-ornithine DABA AT/DC fusion protein Vibrio cholerae serotype O1

Organism

Organism UniProt Comment Textmining
Vibrio cholerae serotype O1
-
 El Tor strain
-
Vibrio cholerae serotype O1 El Tor
-
 El Tor strain
-

Purification (Commentary)

Purification (Comment) Organism
DABA AT/DC affinity-purified Vibrio cholerae serotype O1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2,4-diaminobutyrate DABA AT/DC fusion protein exhibits a preference for L-2,4-diaminobutyrate over L-ornithine by 1000fold Vibrio cholerae serotype O1 ? + CO2
-
 ?
L-2,4-diaminobutyrate DABA AT/DC fusion protein exhibits a preference for L-2,4-diaminobutyrate over L-ornithine by 1000fold Vibrio cholerae serotype O1 El Tor ? + CO2
-
 ?
L-ornithine Kcat/Km is 1000fold lower than with L-2,4-diaminobutyrate Vibrio cholerae serotype O1 1,4-diaminobutane + CO2
-
 ?
L-ornithine Kcat/Km is 1000fold lower than with L-2,4-diaminobutyrate Vibrio cholerae serotype O1 El Tor 1,4-diaminobutane + CO2
-
 ?

Synonyms

Synonyms Comment Organism
DABA aminotransferase/decarboxylase
-
 Vibrio cholerae serotype O1
DABA AT/DC
-
 Vibrio cholerae serotype O1
L-2,4-diaminobutyrate aminotransferase/decarboxylase
-
 Vibrio cholerae serotype O1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.54
-
 L-ornithine DABA AT/DC fusion protein Vibrio cholerae serotype O1
8.3
-
 L-2,4-diaminobutyrate DABA AT//DC fusion protein Vibrio cholerae serotype O1

General Information

General Information Comment Organism
physiological function the DABA AT/DC fusion protein is responsible for diaminopropane production Vibrio cholerae serotype O1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.049
-
 L-ornithine DABA AT/DC fusion protein Vibrio cholerae serotype O1
49
-
 L-2,4-diaminobutyrate DABA AT/DC fusion protein Vibrio cholerae serotype O1