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Literature summary for 4.1.1.86 extracted from
- Purification and characterization of L-2,4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus (1992), J. Gen. Microbiol., 138, 1461-1465.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Acinetobacter calcoaceticus |  |
| Mn2+ | - |
Acinetobacter calcoaceticus | |
| Ni2+ | - |
Acinetobacter calcoaceticus | |
| Zn2+ | - |
Acinetobacter calcoaceticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.51 | - |
L-2,4-diaminobutanoate | pH 8.5, 37°C | Acinetobacter calcoaceticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates, preferred divalent cation | Acinetobacter calcoaceticus | |
| Mg2+ | activates | Acinetobacter calcoaceticus | |
| additional information | no effect by Na+ and K+ | Acinetobacter calcoaceticus | |
| Sr2+ | activates | Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
2 * 53000, SDS-PAGE | Acinetobacter calcoaceticus |
| 108000 | - |
gel filtration | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2,4-diaminobutanoate | Acinetobacter calcoaceticus | - |
propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | Acinetobacter calcoaceticus ATCC 23055 | - |
propane-1,3-diamine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
N-terminal amino acid sequence; strain ATCC23055 | - |
| Acinetobacter calcoaceticus ATCC 23055 | - |
N-terminal amino acid sequence; strain ATCC23055 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 2150fold to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and two steps of gel filtration | Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.2 | - |
purified enzyme | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2,4-diaminobutanoate | - |
Acinetobacter calcoaceticus | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | specific for | Acinetobacter calcoaceticus | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | - |
Acinetobacter calcoaceticus ATCC 23055 | propane-1,3-diamine + CO2 | - |
? | |
| L-2,4-diaminobutanoate | specific for | Acinetobacter calcoaceticus ATCC 23055 | propane-1,3-diamine + CO2 | - |
? | |
| additional information | no activity with 4-N-acetyl-L-2,4-diaminobutanoate, L-2,3-diaminopropionate, L-ornithine, and L-lysine | Acinetobacter calcoaceticus | ? | - |
? | |
| additional information | no activity with 4-N-acetyl-L-2,4-diaminobutanoate, L-2,3-diaminopropionate, L-ornithine, and L-lysine | Acinetobacter calcoaceticus ATCC 23055 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DABA DC | - |
Acinetobacter calcoaceticus |
| DABA decarboxylase | - |
Acinetobacter calcoaceticus |
| L-2,4-diaminobutyrate decarboxylase | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Acinetobacter calcoaceticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, stable for more than 15 min at pH 8.5 | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 8.8 | - |
Acinetobacter calcoaceticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 9.3 | half maximal activity at pH 7.8 and pH 9.3 | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | maximal activity at 0.1 mM PLP, depletion leads to irreversible loss of 70% activity | Acinetobacter calcoaceticus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Acinetobacter calcoaceticus | isoelectric focusing | - |
5 |
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