Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme complexed with L-gulonate 6-phosphate, L-threonohydroxamate 4-phosphate, and L-xylitol 5-phosphate, analogues of the substrate, enediolate intermediate, and product, as well as with the product L-xylulose 5-phosphate, 15 mg/ml protein in 50 mM HEPES, pH 7.5, 5 mM MgCl2, 100 mM NaCl, micro-batch method, 0.01 ml protein solution mixed with equal volume of crystallization solution containing 16% monomethyl PEG 5000, 100 mM Bis-Tris propane, pH 7.0, and 5 mM MgCl2, with 25 mM ligand, X-ray diffraction structure determination and analysis at 1.2, 1.8, 1.7, and 1.8 A resolution, respectively | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-xylitol 5-phosphate | binding structure | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ | Escherichia coli | - |
L-xylulose 5-phosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P39304 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ = L-xylulose 5-phosphate + CO2 | active site structure, substrate binding structure, formation of a cis-1,2-enediolate anion intermediate, intermediate structure and reaction mechanism involving residues Lys64, Asp67, and His136, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydro-L-gulonate 6-phosphate + H+ | - |
Escherichia coli | L-xylulose 5-phosphate + CO2 | - |
? | |
3-dehydro-L-gulonate 6-phosphate + H+ | substrate binding structure | Escherichia coli | L-xylulose 5-phosphate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-keto-L-gulonate 6-phosphate decarboxylase | - |
Escherichia coli |
KGPDC | - |
Escherichia coli |
More | the enzyme belongs to the orotidine 5'-monophosphate decarboxylase OMPDC suprafamily, overview | Escherichia coli |