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Literature summary for 4.1.1.82 extracted from

  • Nakashita, H.; Watanabe, K.; Hara, O.; Hidaka, T.; Seto, H.
    Studies on the biosynthesis of bialaphos. Biochemical mechanism of C-P bond formation: discovery of phosphonopyruvate decarboxylase which catalyzes the formation of phosphonoacetaldehyde from phosphonopyruvate (1997), J. Antibiot., 50, 212-219.
    View publication on PubMed
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Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 4 * 36000, SDS-PAGE Streptomyces hygroscopicus
135000
-
 gel filtration Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phosphonopyruvate Streptomyces hygroscopicus
-
 2-phosphonoacetaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, TSK-gel Phenyl-5PW, gel filtration, Mono Q Streptomyces hygroscopicus

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 7.5, 3 weeks, no loss of activity Streptomyces hygroscopicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phosphonopyruvate
-
 Streptomyces hygroscopicus 2-phosphonoacetaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
PnPy decarboxylase
-
 Streptomyces hygroscopicus

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Streptomyces hygroscopicus