Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Bordetella bronchiseptica |
Protein Variants | Comment | Organism |
---|---|---|
G74C | the mutant of arylmalonate decarboxylase from Bordatella bronchoseptica has a racemising activity towards profens | Bordetella bronchiseptica |
G74C/G190E | inactive | Bordetella bronchiseptica |
G74C/G190H | inactive | Bordetella bronchiseptica |
G74C/M159L | the mutant shows 14% decarboxylation activity and 165% racemisation activity compared to mutant G74C | Bordetella bronchiseptica |
G74C/P14D | inactive | Bordetella bronchiseptica |
G74C/V13D | inactive | Bordetella bronchiseptica |
G74C/V13D/G190H | inactive | Bordetella bronchiseptica |
G74C/V43A | the mutant with shows a 20fold shift towards promiscuous racemisation based on a reduced activity in the decarboxylation reaction and a 2fold increase in the racemisation activity. The mutant shows an extended substrate range, with a 30fold increase in the reaction rate towards ketoprofen | Bordetella bronchiseptica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.4 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
11.4 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/V43A, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
18.3 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/M159L, pH and temperature not specified in the publication | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | Q05115 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography, gel filtration | Bordetella bronchiseptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica | 2-arylpropionate + CO2 | - |
? | |
additional information | 2-methyl-2-(4-isobutylphenyl)malonic acid is not a substrate of wild type AMDase | Bordetella bronchiseptica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/V43A, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.063 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/M159L, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.27 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C, pH and temperature not specified in the publication | Bordetella bronchiseptica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/V43A, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.0034 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C/M159L, pH and temperature not specified in the publication | Bordetella bronchiseptica | |
0.026 | - |
2-aryl-2-methylmalonate | mutant enzyme G74C, pH and temperature not specified in the publication | Bordetella bronchiseptica |