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Literature summary for 4.1.1.76 extracted from

  • Obata, R.; Nakasako, M.
    Structural basis for inverting the enantioselectivity of arylmalonate decarboxylase revealed by the structural analysis of the Gly74Cys/Cys188Ser mutant in the liganded form (2010), Biochemistry, 49, 1963-1969.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bordetella bronchiseptica

Crystallization (Commentary)

Crystallization (Comment) Organism
G74C/C188S mutant in the liganded form at a resolution of 1.45 A, hanging drop vapor diffusion method, using 1.2 M ammonium sulfate, 15% (w/v) PEG5000, 10 mM bromophenylacetate, 1% (v/v) dioxane, and 70 mM HEPES (pH 7.6) Bordetella bronchiseptica

Protein Variants

Protein Variants Comment Organism
G74C/C188S the mutation inverts the enantioselectivity of the enzyme, the mutations have little effect on the structure of the active site Bordetella bronchiseptica

Organism

Organism UniProt Comment Textmining
Bordetella bronchiseptica Q05115
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bordetella bronchiseptica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aryl-2-methylmalonate
-
Bordetella bronchiseptica (2R)-arylpropionate + CO2
-
?

Synonyms

Synonyms Comment Organism
AMDase
-
Bordetella bronchiseptica