Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Bordetella bronchiseptica |
Crystallization (Comment) | Organism |
---|---|
G74C/C188S mutant in the liganded form at a resolution of 1.45 A, hanging drop vapor diffusion method, using 1.2 M ammonium sulfate, 15% (w/v) PEG5000, 10 mM bromophenylacetate, 1% (v/v) dioxane, and 70 mM HEPES (pH 7.6) | Bordetella bronchiseptica |
Protein Variants | Comment | Organism |
---|---|---|
G74C/C188S | the mutation inverts the enantioselectivity of the enzyme, the mutations have little effect on the structure of the active site | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | Q05115 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bordetella bronchiseptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica | (2R)-arylpropionate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |