Protein Variants | Comment | Organism |
---|---|---|
G190A | the mutant shows 4.6% relative activity compared to the wild type enzyme | Bordetella bronchiseptica |
G190S/P14V/P15G | the mutant shows 1.9% relative activity compared to the wild type enzyme | Bordetella bronchiseptica |
M159C | the mutant shows 1.4% relative activity compared to the wild type enzyme using phenylmalonate or methyl(phenyl)propanedioic acid as substrate, the mutant shows 1.7% relative activity compared to the wild type enzyme using 2-hydroxy-2-phenylmalonic acid as substrate | Bordetella bronchiseptica |
M159G | the mutant shows 19% relative activity compared to the wild type enzyme | Bordetella bronchiseptica |
M159S | the mutant shows 2.6% relative activity compared to the wild type enzyme | Bordetella bronchiseptica |
M159V | the mutant shows 51% relative activity compared to the wild type enzyme | Bordetella bronchiseptica |
P14V/P15G | the mutant shows 11% relative activity compared to the wild type enzyme using phenylmalonate as substrate, the mutant shows 1.9% relative activity compared to the wild type enzyme using 2-hydroxy-2-phenylmalonic acid as substrate, the mutant shows 1.5% relative activity compared to the wild type enzyme using hydroxy(2-methylprop-1-en-1-yl)propanedioic acid as substrate | Bordetella bronchiseptica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzylphosphonate | competitive inhibitor of AMDase | Bordetella bronchiseptica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Phenylmalonate | mutant enzyme M159V | Bordetella bronchiseptica | |
0.43 | - |
Phenylmalonate | mutant enzyme M159G | Bordetella bronchiseptica | |
0.8 | - |
2-vinyl-2-methylmalonic acid | mutant enzyme G190A | Bordetella bronchiseptica | |
3.5 | - |
methyl(2-methylprop-1-en-1-yl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
3.5 | - |
Phenylmalonate | mutant enzyme P14V/P15G | Bordetella bronchiseptica | |
4.1 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme M159C | Bordetella bronchiseptica | |
5 | - |
Phenylmalonate | mutant enzyme M159S | Bordetella bronchiseptica | |
7.3 | - |
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
7.8 | - |
2-vinyl-2-methylmalonic acid | wild type enzyme | Bordetella bronchiseptica | |
9.1 | - |
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
10.7 | - |
Phenylmalonate | wild type enzyme | Bordetella bronchiseptica | |
12.1 | - |
Phenylmalonate | mutant enzyme M159C | Bordetella bronchiseptica | |
12.8 | - |
methyl[(1E)-prop-1-en-1-yl]propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
14.3 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme G190S/P14V/P15G | Bordetella bronchiseptica | |
14.4 | - |
(1E)-but-1-en-1-yl(methyl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
15.3 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme P14V/P15G | Bordetella bronchiseptica | |
26.9 | - |
methyl(phenyl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
30.2 | - |
2-hydroxy-2-phenylmalonic acid | wild type enzyme | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | Q05115 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1E)-but-1-en-1-yl(methyl)propanedioic acid | - |
Bordetella bronchiseptica | (2S,3Z)-2-methylhex-3-enoic acid + CO2 | - |
? | |
2-amino-2-vinylmalonate | - |
Bordetella bronchiseptica | (R)-vinylglycine + CO2 | - |
? | |
2-hydroxy-2-phenylmalonic acid | - |
Bordetella bronchiseptica | (2S)-hydroxy(phenyl)ethanoic acid + CO2 | - |
? | |
2-hydroxy-2-phenylmalonic acid | - |
Bordetella bronchiseptica | ? + CO2 | - |
? | |
2-vinyl-2-methylmalonic acid | - |
Bordetella bronchiseptica | (2S)-2-methylbut-3-enoic acid + CO2 | - |
? | |
2-vinyl-2-methylmalonic acid | - |
Bordetella bronchiseptica | ? + CO2 | - |
? | |
amino(phenyl)propanedioic acid | - |
Bordetella bronchiseptica | (2S)-amino(phenyl)ethanoic acid + CO2 | - |
? | |
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid | - |
Bordetella bronchiseptica | (2S)-2-hydroxy-4-methylpent-3-enoic acid + CO2 | - |
? | |
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid | - |
Bordetella bronchiseptica | (2S)-2-hydroxybut-3-enoic acid + CO2 | - |
? | |
methyl(2-methylprop-1-en-1-yl)propanedioic acid | - |
Bordetella bronchiseptica | (2S)-2,4-dimethylpent-3-enoic acid + CO2 | - |
? | |
methyl(phenyl)propanedioic acid | - |
Bordetella bronchiseptica | (2S)-2-phenylpropanoic acid + CO2 | - |
? | |
methyl[(1E)-prop-1-en-1-yl]propanedioic acid | - |
Bordetella bronchiseptica | (2S,3Z)-2-methylpent-3-enoic acid + CO2 | - |
? | |
Phenylmalonate | best substrate | Bordetella bronchiseptica | Phenylacetate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
methyl(2-methylprop-1-en-1-yl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
20.7 | - |
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
20.8 | - |
2-vinyl-2-methylmalonic acid | mutant enzyme G190A | Bordetella bronchiseptica | |
23.4 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme M159C | Bordetella bronchiseptica | |
23.9 | - |
(1E)-but-1-en-1-yl(methyl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
42.8 | - |
2-vinyl-2-methylmalonic acid | wild type enzyme | Bordetella bronchiseptica | |
46.2 | - |
methyl[(1E)-prop-1-en-1-yl]propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
47.6 | - |
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
94.4 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme G190S/P14V/P15G | Bordetella bronchiseptica | |
99.8 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme P14V/P15G | Bordetella bronchiseptica | |
101 | - |
2-hydroxy-2-phenylmalonic acid | wild type enzyme | Bordetella bronchiseptica | |
246 | - |
Phenylmalonate | mutant enzyme M159G | Bordetella bronchiseptica | |
279 | - |
methyl(phenyl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
316 | - |
Phenylmalonate | wild type enzyme | Bordetella bronchiseptica | |
386 | - |
Phenylmalonate | mutant enzyme M159S | Bordetella bronchiseptica | |
450 | - |
Phenylmalonate | mutant enzyme M159V | Bordetella bronchiseptica | |
489 | - |
Phenylmalonate | mutant enzyme M159C | Bordetella bronchiseptica | |
1143 | - |
Phenylmalonate | mutant enzyme P14V/P15G | Bordetella bronchiseptica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | AMDase does not require cofactors | Bordetella bronchiseptica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
benzylphosphonate | - |
Bordetella bronchiseptica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
methyl(2-methylprop-1-en-1-yl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
1.7 | - |
(1E)-but-1-en-1-yl(methyl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
2.8 | - |
hydroxy(2-methylprop-1-en-1-yl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
3.3 | - |
2-hydroxy-2-phenylmalonic acid | wild type enzyme | Bordetella bronchiseptica | |
3.6 | - |
methyl[(1E)-prop-1-en-1-yl]propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
5.2 | - |
hydroxy[(1E)-prop-1-en-1-yl]propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
5.5 | - |
2-vinyl-2-methylmalonic acid | wild type enzyme | Bordetella bronchiseptica | |
5.8 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme M159C | Bordetella bronchiseptica | |
6.5 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme P14V/P15G | Bordetella bronchiseptica | |
6.6 | - |
2-hydroxy-2-phenylmalonic acid | mutant enzyme G190S/P14V/P15G | Bordetella bronchiseptica | |
10.4 | - |
methyl(phenyl)propanedioic acid | wild type enzyme | Bordetella bronchiseptica | |
25.2 | - |
2-vinyl-2-methylmalonic acid | mutant enzyme G190A | Bordetella bronchiseptica | |
29.6 | - |
Phenylmalonate | wild type enzyme | Bordetella bronchiseptica | |
40.4 | - |
Phenylmalonate | mutant enzyme M159C | Bordetella bronchiseptica | |
77.2 | - |
Phenylmalonate | mutant enzyme M159S | Bordetella bronchiseptica | |
326 | - |
Phenylmalonate | mutant enzyme P14V/P15G | Bordetella bronchiseptica | |
570 | - |
Phenylmalonate | mutant enzyme M159G | Bordetella bronchiseptica | |
1499 | - |
Phenylmalonate | mutant enzyme M159V | Bordetella bronchiseptica |