Application | Comment | Organism |
---|---|---|
biotechnology | enzyme is of biotechnological interest for its use in the synthesis of fine chemicals | Bordetella bronchiseptica |
Cloned (Comment) | Organism |
---|---|
complete gene is cloned into pBAD His A vector, and Escherichia coli Top10 cells are transformed | Bordetella bronchiseptica |
Crystallization (Comment) | Organism |
---|---|
vapour-diffusion hanging-drop setup | Bordetella bronchiseptica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25940 | - |
freshly purified AMDase | Bordetella bronchiseptica |
25950 | - |
calculated molecular mass, including the C-terminal His-tag | Bordetella bronchiseptica |
26030 | - |
crystals of AMDase can only be obtained after storage of the protein stock solution for about 4-5 weeks at 277 K. Electron spray ionization mass spectrometry measurement of a crystallizable AMDase sample shows two main components: 26025 (32%) and 26101 Da (68%), which are 83 and 159 Da higher than that of freshly purified AMDase (25942 Da) | Bordetella bronchiseptica |
26100 | - |
crystals of AMDase can only be obtained after storage of the protein stock solution for about 4-5 weeks at 277 K. Electron spray ionization mass spectrometry measurement of a crystallizable AMDase sample shows two main components: 26025 (32%) and 26101 Da (68%), which are 83 and 159 Da higher than that of freshly purified AMDase (25942 Da) | Bordetella bronchiseptica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aryl-2-methylmalonate | Bordetella bronchiseptica | - |
2-arylpropionate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | Q05115 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity chromatography | Bordetella bronchiseptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica | 2-arylpropionate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Arylmalonate decarboxylase | - |
Bordetella bronchiseptica |