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Literature summary for 4.1.1.76 extracted from
- Crystallization and preliminary X-ray diffraction experiments of arylmalonate decarboxylase from Alcaligenes bronchisepticus (2008), Acta Crystallogr. Sect. F, 64, 610-613.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli strain DH5alpha-MCR | Bordetella bronchiseptica |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapour-diffusion method, crystallized with ammonium sulfate under alkaline pH conditions with the aim of understanding the mechanism of the enantioselective reaction | Bordetella bronchiseptica |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G74C/C188S | mutated AMDase produces arylpropionate of the opposite enantiomorph to that of the wild-type enzyme, although the enzymatic reaction proceeds with a slower rate than that of the wild type | Bordetella bronchiseptica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24740 | - |
electrospray ion mass spectrometry | Bordetella bronchiseptica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aryl-2-methylmalonate | Bordetella bronchiseptica | - |
2-arylpropionate + CO2 | optically active | ? |  |
| 2-aryl-2-methylmalonate | Bordetella bronchiseptica KU1201 | - |
2-arylpropionate + CO2 | optically active | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bordetella bronchiseptica | Q05115 | - |
- |
| Bordetella bronchiseptica KU1201 | Q05115 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bordetella bronchiseptica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica | 2-arylpropionate + CO2 | optically active | ? | |
| 2-aryl-2-methylmalonate | - |
Bordetella bronchiseptica KU1201 | 2-arylpropionate + CO2 | optically active | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Bordetella bronchiseptica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMDase | - |
Bordetella bronchiseptica |
| Arylmalonate decarboxylase | - |
Bordetella bronchiseptica |
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Release 2023.1 (January 2023)
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