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Literature summary for 4.1.1.76 extracted from

  • Terao, Y.; Ijima, Y.; Miyamoto, K.; Ohta, H.
    Inversion of enantioselectivity of arylmalonate decarboxylase via site-directed mutation based on the proposed reaction mechanism (2007), J. Mol. Catal. B, 45, 15-20.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
A68C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica
C188S mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme Bordetella bronchiseptica
G74C/C188S mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme Bordetella bronchiseptica
L72C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica
L77C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica
M73C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica
S71C/C188S mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme Bordetella bronchiseptica
S76C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica
T75C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica
V69C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica
V70C/C188S no specific activity using phenylmalonate as a substrate Bordetella bronchiseptica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
alpha-methyl-alpha-(beta-naphthyl)malonate wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
0.51
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
1.01
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
1.25
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme G74C/S188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
5.03
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
12.5
-
alpha-methyl-alpha-(alpha-thienyl)malonate wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica

Organism

Organism UniProt Comment Textmining
Bordetella bronchiseptica
-
-
-
Bordetella bronchiseptica KU1201
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography and Butyl-Toyopearl column chromatography Bordetella bronchiseptica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
mutant enzyme G74C/C188S, pH 8.5, 37°C Bordetella bronchiseptica
0.07
-
mutant enzyme S71C/C188S, pH 8.5, 37°C Bordetella bronchiseptica
1.3
-
mutant enzyme C188S, pH 8.5, 37°C Bordetella bronchiseptica
467
-
wild type enzyme, pH 8.5, 37°C Bordetella bronchiseptica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-methyl-alpha-(alpha-thienyl)malonate
-
Bordetella bronchiseptica alpha-(alpha-thienyl)propionate + CO2
-
?
alpha-methyl-alpha-(alpha-thienyl)malonate
-
Bordetella bronchiseptica KU1201 alpha-(alpha-thienyl)propionate + CO2
-
?
alpha-methyl-alpha-(beta-naphthyl)malonate
-
Bordetella bronchiseptica alpha-(beta-naphthyl) propionate + CO2
-
?
alpha-methyl-alpha-(beta-naphthyl)malonate
-
Bordetella bronchiseptica KU1201 alpha-(beta-naphthyl) propionate + CO2
-
?
Phenylmalonate
-
Bordetella bronchiseptica Phenylacetate + CO2
-
?
Phenylmalonate
-
Bordetella bronchiseptica KU1201 Phenylacetate + CO2
-
?

Synonyms

Synonyms Comment Organism
AMDase
-
Bordetella bronchiseptica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
0.08
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
0.12
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme G74C/S188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
0.34
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
30.9
-
alpha-methyl-alpha-(beta-naphthyl)malonate wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica
200
-
alpha-methyl-alpha-(alpha-thienyl)malonate wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C Bordetella bronchiseptica