Protein Variants | Comment | Organism |
---|---|---|
A68C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
C188S | mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme | Bordetella bronchiseptica |
G74C/C188S | mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme | Bordetella bronchiseptica |
L72C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
L77C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
M73C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
S71C/C188S | mutant exhibits decarboxylation activity and gives the opposite enantiomer to that formed by the wild type enzyme | Bordetella bronchiseptica |
S76C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
T75C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
V69C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
V70C/C188S | no specific activity using phenylmalonate as a substrate | Bordetella bronchiseptica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
0.51 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
1.01 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
1.25 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme G74C/S188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
5.03 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
12.5 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bordetella bronchiseptica | - |
- |
- |
Bordetella bronchiseptica KU1201 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography and Butyl-Toyopearl column chromatography | Bordetella bronchiseptica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.05 | - |
mutant enzyme G74C/C188S, pH 8.5, 37°C | Bordetella bronchiseptica |
0.07 | - |
mutant enzyme S71C/C188S, pH 8.5, 37°C | Bordetella bronchiseptica |
1.3 | - |
mutant enzyme C188S, pH 8.5, 37°C | Bordetella bronchiseptica |
467 | - |
wild type enzyme, pH 8.5, 37°C | Bordetella bronchiseptica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-methyl-alpha-(alpha-thienyl)malonate | - |
Bordetella bronchiseptica | alpha-(alpha-thienyl)propionate + CO2 | - |
? | |
alpha-methyl-alpha-(alpha-thienyl)malonate | - |
Bordetella bronchiseptica KU1201 | alpha-(alpha-thienyl)propionate + CO2 | - |
? | |
alpha-methyl-alpha-(beta-naphthyl)malonate | - |
Bordetella bronchiseptica | alpha-(beta-naphthyl) propionate + CO2 | - |
? | |
alpha-methyl-alpha-(beta-naphthyl)malonate | - |
Bordetella bronchiseptica KU1201 | alpha-(beta-naphthyl) propionate + CO2 | - |
? | |
Phenylmalonate | - |
Bordetella bronchiseptica | Phenylacetate + CO2 | - |
? | |
Phenylmalonate | - |
Bordetella bronchiseptica KU1201 | Phenylacetate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Bordetella bronchiseptica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
0.08 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
0.12 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | mutant enzyme G74C/S188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
0.34 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
30.9 | - |
alpha-methyl-alpha-(beta-naphthyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica | |
200 | - |
alpha-methyl-alpha-(alpha-thienyl)malonate | wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C | Bordetella bronchiseptica |