Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Achromobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
SDS-PAGE | Achromobacter sp. |
24740 | - |
calculated from amino acid sequence | Achromobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter sp. | - |
- |
- |
Achromobacter sp. KU1311 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, DEAE-Toyopearl column chromatography, and Butyl-Toyopearl column chromatography | Achromobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
crude cell extract | Achromobacter sp. |
380 | - |
after 528fold purification | Achromobacter sp. |
Storage Stability | Organism |
---|---|
4°C, 100 mM Tris-HCl pH 8.0, 2 months, 50% loss of activity | Achromobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl(phenyl)propanedioate | - |
Achromobacter sp. | (2R)-2-phenylpropanoate + CO2 | - |
? | |
methyl(phenyl)propanedioate | - |
Achromobacter sp. KU1311 | (2R)-2-phenylpropanoate + CO2 | - |
? | |
methyl(thiophen-2-yl)propanedioate | - |
Achromobacter sp. | (2S)-2-thiophen-2-ylpropanoate + CO2 | - |
? | |
methyl(thiophen-2-yl)propanedioate | - |
Achromobacter sp. KU1311 | (2S)-2-thiophen-2-ylpropanoate + CO2 | - |
? | |
additional information | not active with (2S)-2-methyl-2-phenylbutanoic acid | Achromobacter sp. | ? | - |
? | |
additional information | not active with (2S)-2-methyl-2-phenylbutanoic acid | Achromobacter sp. KU1311 | ? | - |
? | |
Phenylmalonate | phenylmalonate is phenylpropanedioate | Achromobacter sp. | Phenylacetate + CO2 | - |
? | |
Phenylmalonate | phenylmalonate is phenylpropanedioate | Achromobacter sp. KU1311 | Phenylacetate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AMDase | - |
Achromobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Achromobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | activity decreases to 70% of the maximum at 25°C and 50°C | Achromobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | retains 100% activity at 30°C, activity is almost negligible at 60°C | Achromobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Achromobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Achromobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
activity markedly decreases above pH 8.5 | Achromobacter sp. |