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Literature summary for 4.1.1.76 extracted from

  • Terao, Y.; Miyamoto, K.; Ohta, H.
    Improvement of the activity of arylmalonate decarboxylase by random mutagenesis (2006), Appl. Microbiol. Biotechnol., 73, 647-653.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Bordetella bronchiseptica

Protein Variants

Protein Variants Comment Organism
G74C/C188S very low activity Bordetella bronchiseptica
S36N activity is about 1/10 compared to that of the wild-type enzyme Bordetella bronchiseptica
S36N/G74C/C188S 10fold increased activity than the G74C/C188S double mutant enzyme Bordetella bronchiseptica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
alpha-methyl-alpha-(beta-naphthyl)malonate wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
0.52
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
1.11
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
1.25
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
3.51
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
5.03
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
12.5
-
alpha-methyl-alpha-(alpha-thienyl)malonate wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
14.3
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica

Organism

Organism UniProt Comment Textmining
Bordetella bronchiseptica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
diethylamino ethanol-Toyopearl column chromatography and Butyl-Toyopearl column chromatography Bordetella bronchiseptica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-methyl-alpha-(alpha-thienyl)malonate
-
Bordetella bronchiseptica alpha-(alpha-thienyl)propionate + CO2
-
?
alpha-methyl-alpha-(beta-naphthyl)malonate
-
Bordetella bronchiseptica alpha-(beta-naphthyl) propionate + CO2
-
?
Phenylmalonate
-
Bordetella bronchiseptica Phenylacetate + CO2
-
?

Synonyms

Synonyms Comment Organism
AMDase
-
Bordetella bronchiseptica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
0.34
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
1.14
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
3.09
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme S36N/G74C/C188S, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
3.7
-
alpha-methyl-alpha-(beta-naphthyl)malonate mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
18.9
-
alpha-methyl-alpha-(alpha-thienyl)malonate mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
30.9
-
alpha-methyl-alpha-(beta-naphthyl)malonate wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica
200
-
alpha-methyl-alpha-(alpha-thienyl)malonate wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C Bordetella bronchiseptica