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Literature summary for 4.1.1.75 extracted from

  • Vanderbilt, A.S.; Gaby, N.S.; Rodwell, V.W.
    Intermediates and enzymes between alpha-ketoarginine and gamma-guanidinobutyrate in the L-arginine catabolic pathway of Pseudomonas putida (1975), J. Biol. Chem., 250, 5322-5329.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alpha-Hydroxy-delta-guanidino-N-pentanoate weak Pseudomonas putida
delta-Guanidinopentanoate
-
Pseudomonas putida
epsilon-Guanidinohexanoate
-
Pseudomonas putida
Ethylguanidine
-
Pseudomonas putida
gamma-Guanidinobutanoate weak Pseudomonas putida
gamma-guanidinobutyraldehyde
-
Pseudomonas putida
Guanidinoacetate weak Pseudomonas putida
Hexylguanidine
-
Pseudomonas putida
L-Arg weak Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ activates Pseudomonas putida
Ni2+ activates Pseudomonas putida
Mn2+ activates, half-maximal activity at 0.03 mM Pseudomonas putida
Mg2+ activates, half-maximal activity at 0.3 mM Pseudomonas putida
Co2+ activates, half-maximal activity at 0.3 mM Pseudomonas putida
Ca2+ activates, half-maximal activity at 0.6 mM Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-Guanidino-2-oxopentanoate Pseudomonas putida induced by Arg or gamma-guanidinobutyraldehyde ?
-
?
5-Guanidino-2-oxopentanoate Pseudomonas putida P2 induced by Arg or gamma-guanidinobutyraldehyde ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida P2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-Guanidino-2-oxopentanoate i.e. alpha-ketoarginine Pseudomonas putida 4-Guanidinobutyraldehyde + CO2
-
?
5-Guanidino-2-oxopentanoate i.e. alpha-ketoarginine Pseudomonas putida P2 4-Guanidinobutyraldehyde + CO2
-
?
5-Guanidino-2-oxopentanoate induced by Arg or gamma-guanidinobutyraldehyde Pseudomonas putida ?
-
?
5-Guanidino-2-oxopentanoate induced by Arg or gamma-guanidinobutyraldehyde Pseudomonas putida P2 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
30 min, stable Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
167
-
5-guanidino-2-oxopentanoate
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ required Pseudomonas putida
thiamine diphosphate required Pseudomonas putida