Protein Variants | Comment | Organism |
---|---|---|
D29E | significant decrease in Km-values | Enterobacter cloacae |
E468D | slight decrease in Km-values | Enterobacter cloacae |
E52D | decrease in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, increase in Km-value for benzoylformic acid | Enterobacter cloacae |
H115K | slight increase in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, decrease in Km-value for benzoylformic acid | Enterobacter cloacae |
Q383T | decrease in Km-value for pyruvate | Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
3-(Indol-3-yl)pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
0.018 | - |
3-(Indol-3-yl)pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
0.02 | - |
3-(Indol-3-yl)pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
0.024 | - |
benzoylformic acid | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
0.024 | - |
3-(Indol-3-yl)pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
0.032 | - |
3-(Indol-3-yl)pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
0.14 | - |
benzoylformic acid | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
0.27 | - |
benzoylformic acid | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
0.63 | - |
pyruvate | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
0.73 | - |
pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
0.77 | - |
pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
1.65 | - |
benzoylformic acid | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
1.71 | - |
benzoylformic acid | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
2.72 | - |
benzoylformic acid | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
3.16 | - |
pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
3.38 | - |
pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
3.48 | - |
pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 | residues E468, D29, and H115 are involved in binding of all substrates and decarboxlation of the respective tetrahedral thiamine-diphosphate-substrate adducts. Residue E383 is central to substrate specificity | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(indol-3-yl)pyruvate | - |
Enterobacter cloacae | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
benzoylformic acid | - |
Enterobacter cloacae | benzaldehyde + CO2 | - |
? | |
pyruvate | - |
Enterobacter cloacae | acetaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ipyr | - |
Enterobacter cloacae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
benzoylformic acid | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
0.016 | - |
pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
0.019 | - |
pyruvate | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
0.04 | - |
3-(Indol-3-yl)pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
0.06 | - |
pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
0.069 | - |
pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
0.07 | - |
benzoylformic acid | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
0.1 | - |
3-(Indol-3-yl)pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
0.29 | - |
benzoylformic acid | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
0.31 | - |
3-(Indol-3-yl)pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
0.87 | - |
benzoylformic acid | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
0.88 | - |
pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
0.98 | - |
3-(Indol-3-yl)pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
2.05 | - |
3-(Indol-3-yl)pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
2.06 | - |
pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
2.25 | - |
benzoylformic acid | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
11.6 | - |
benzoylformic acid | wild-type, pH 6.5, 30°C | Enterobacter cloacae |