Cloned (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
General Stability | Organism |
---|---|
less stable at a high potassium phosphate concentration | Enterobacter cloacae |
slightly stabilized in presence of 5 mM MgCl2 | Enterobacter cloacae |
stabilized by addition of 1 mM thiamine diphosphate | Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutaric acid | - |
Enterobacter cloacae | |
3-phenylpyruvic acid | competitive against indole-3-acetic acid | Enterobacter cloacae | |
Pyruvic acid | - |
Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
3-(Indol-3-yl)pyruvate | - |
Enterobacter cloacae | |
2.5 | - |
Pyruvic acid | - |
Enterobacter cloacae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, 10fold increase in activity. 39fold increase in activity in presence of 0.1 mM thiamine diphosphate and 5 mM Mg2+. One of the roles of Mg2+ is to facilitate the binding of thiamin diphosphate to the enzyme by formation of a thiamine diphosphate complex | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Enterobacter cloacae |
240000 | - |
gel filtration | Enterobacter cloacae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(Indol-3-yl)pyruvate | Enterobacter cloacae | key enzyme for indole-3-acetic acid biosynthesis | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | rather stable in ethanol | Enterobacter cloacae |
Ethyl acetate | rather stable in ethyl acetate | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli cells | Enterobacter cloacae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(Indol-3-yl)pyruvate | - |
Enterobacter cloacae | Indole-3-acetaldehyde + CO2 | - |
? | |
3-(Indol-3-yl)pyruvate | key enzyme for indole-3-acetic acid biosynthesis | Enterobacter cloacae | ? | - |
? | |
pyruvic acid | decarboxylation at 19% of the indole-3-acetic acid decarboxylation | Enterobacter cloacae | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, SDS-PAGE | Enterobacter cloacae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
24 h, in presence of 1 mM thiamine diphosphate and 5 mM MgCl2, stable | Enterobacter cloacae |
50 | - |
30 min, in presence of 1 mM thiamine diphosphate and 5 mM MgCl2, stable | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.6 | - |
Enterobacter cloacae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | 0.1 mM, 8fold increase in activity. 39fold increase in activity in presence of 0.1 mM thiamine diphosphate and 5 mM Mg2+ | Enterobacter cloacae |