Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli SG13009 cells | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
A460I | the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity | Pseudomonas putida |
A460I/F464I | the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity | Pseudomonas putida |
F464I | the mutant exhibits an increased (R)-2-hydroxypropiophenone selectivity | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoylformate | Pseudomonas putida | - |
benzaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + acetaldehyde | reactions performed at high benzaldehyde concentrations and at high hydrostatic pressures show an increase in (R)-2-hydroxypropiophenone ((R)-2-HPP) formation catalyzed by BFD variants F464I, A460I, and A460I/F464I. For BFD mutant A460I/F464I there is an increase in the ee of (R)-2-HPP up to 80%, whereas at atmospheric conditions this variant synthesizes (R)-2-HPP with an ee of only 50%. Alkaline conditions (up to pH 8.5) and high hydrostatic pressures result in an increase of (R)-2-HPP synthesis, especially in the case of variant A460I and F464I | Pseudomonas putida | (R)-2-hydroxypropiophenone | - |
? | |
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
BFD | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Pseudomonas putida |