Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli SG13009 cells | Pseudomonas putida |
General Stability | Organism |
---|---|
the activity of the spherical silica-immobilized BFD A460I-F464I variant is determined to be 70% related to the activity of the free enzyme | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.4 | - |
benzaldehyde | free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C | Pseudomonas putida | |
51.9 | - |
benzaldehyde | immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C | Pseudomonas putida | |
515.9 | - |
acetaldehyde | immobilized mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C | Pseudomonas putida | |
775 | - |
acetaldehyde | free mutant enzyme A460I/F464I, in 50 mM phosphate buffer at pH 7.5 and 20°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20906 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Superdex 200 gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + acetaldehyde | - |
Pseudomonas putida | 2-hydroxypropiophenone | - |
? | |
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
BFD | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
temperature optimum for immobilized variant A460I/F464I | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas putida |