Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain SG13009 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
A460I | for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 | Pseudomonas putida |
A460I/F464I | for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 | Pseudomonas putida |
F464I | for this variant the highest amounts of (S)-2-hydroxypropiophenone-product can be detected at low benzaldehyde concentrations and slightly acidic conditions at pH 6.5 | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Superdex 200 gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + acetaldehyde | - |
Pseudomonas putida | (S)-2-hydroxypropiophenone | - |
? | |
benzylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFD | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Pseudomonas putida |