Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol 2000 and 0.2 M MgCl2, at pH 6. | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-mandelate | - |
Pseudomonas putida | |
methyl benzoylphosphonate | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
p-nitro-benzoylformate | at 25°C | Pseudomonas putida | |
0.23 | - |
benzoylformate | in 50 mM potassium phosphate buffer, pH 6.5, at 30°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20906 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
benzylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
p-nitro-benzoylformate | very poor substrate | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
BFD | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
p-nitro-benzoylformate | at 25°C | Pseudomonas putida | |
426 | - |
benzoylformate | at 30°C, in 50 mM potassium phosphate buffer, pH 6.5 | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas putida |