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Literature summary for 4.1.1.7 extracted from

  • Brandt, G.S.; Kneen, M.M.; Chakraborty, S.; Baykal, A.T.; Nemeria, N.; Yep, A.; Ruby, D.I.; Petsko, G.A.; Kenyon, G.L.; McLeish, M.J.; Jordan, F.; Ringe, D.
    Snapshot of a reaction intermediate: analysis of benzoylformate decarboxylase in complex with a benzoylphosphonate inhibitor (2009), Biochemistry, 48, 3247-3257.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.7

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with methyl benzoylphosphonate, hanging drop vapor diffusion method, using 100 mM Tris-HCl (pH 8.5), 150 mM CaCl2, 22% (v/v) PEG 400 Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
benzoylphosphonate the reaction of methyl benzoylphosphonate with BFDC results in the formation of a stable analogue (C2alpha-phosphonomandelyl thiamine diphosphate) of the covalent thiamine diphosphate-substrate adduct C2alpha-mandelyl thiamine diphosphate Pseudomonas putida
methyl benzoylphosphonate competitive inhibitor Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 methyl benzoylphosphonate
-
 Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P20906
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzaldehyde + acetaldehyde
-
 Pseudomonas putida (S)-2-hydroxypropiophenone
-
 r
benzaldehyde + benzaldehyde
-
 Pseudomonas putida (R)-benzoin
-
 r
methyl benzoylphosphonate
-
 Pseudomonas putida ?
-
 ?

Synonyms

Synonyms Comment Organism
BFDC
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.38
-
 methyl benzoylphosphonate
-
 Pseudomonas putida