Crystallization (Comment) | Organism |
---|---|
mutant enzyme L461A, hanging drop vapour diffusion method | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
A460G | mutant exhibits a higher Km value compared to the wild type enzyme | Pseudomonas putida |
L461A | the mutant catalyzes the S-stereoselective addition of larger acceptor aldehydes, such as propanal with benzaldehyde and its derivatives (a reaction not catalyzed by the wild type enzyme) | Pseudomonas putida |
L461G | the mutant catalyzes the S-stereoselective addition of larger acceptor aldehydes, such as propanal with benzaldehyde and its derivatives (a reaction not catalyzed by the wild type enzyme) | Pseudomonas putida |
L461V | mutant exhibits a higher Km value compared to the wild type enzyme | Pseudomonas putida |
P24A | mutant exhibits a higher Km value compared to the wild type enzyme | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
benzoylformate | mutant enzyme L461V, in potassium phosphate buffer (pH 6.5, 50 mM) | Pseudomonas putida | |
0.25 | - |
benzoylformate | mutant enzyme L461A, in potassium phosphate buffer (pH 6.5, 50 mM) | Pseudomonas putida | |
0.37 | - |
benzoylformate | wild type enzyme, in potassium phosphate buffer (pH 6.5, 50 mM) | Pseudomonas putida | |
0.51 | - |
benzoylformate | mutant enzyme P24A, in potassium phosphate buffer (pH 6.5, 50 mM) | Pseudomonas putida | |
1.4 | - |
benzoylformate | mutant enzyme L461G, in potassium phosphate buffer (pH 6.5, 50 mM) | Pseudomonas putida | |
1.54 | - |
benzoylformate | mutant enzyme A460G, in potassium phosphate buffer (pH 6.5, 50 mM) | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20906 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex G200 gel filtration | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + acetaldehyde | - |
Pseudomonas putida | (S)-2-hydroxy-1-phenylpropan-1-one | - |
? | |
benzaldehyde + benzaldehyde | - |
Pseudomonas putida | (R)-benzoin | - |
? | |
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
additional information | the S-stereoselective addition of larger acceptor aldehydes, such as propanal with benzaldehyde and its derivatives is not catalyzed by the wild type enzyme, but with mutant enzyme L461A | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFD | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas putida |