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Literature summary for 4.1.1.65 extracted from
- Purification and properties of phosphatidylserine decarboxylase from Escherichia coli (1974), J. Biol. Chem., 249, 3079-3084.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| nonionic detergents | e.g. Triton X-100, absolute requirement | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ionic detergents | e.g., Barlox-12, SDS | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound to | Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36500 | - |
x * 36500, SDS-PAGE | Escherichia coli |
| 60000 | 170000 | sucrose density gradient centrifugation, in presence of Triton | Escherichia coli |
| 170000 | - |
sucrose density gradient centrifugation in absence of Triton | Escherichia coli |
| 400000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidyl-L-serine | Escherichia coli | the enzyme catalyzes the final step in the biosynthesis of phosphatidylethanolamine | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 49 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, pH 6.0-7.8, 10% glycerol, 0.1% Triton X-100, stable for 1.5 years. 0°C, stable for several months. Room temperature, stable for at least 1 week | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Phosphatidyl-L-serine | - |
Escherichia coli | Phosphatidylethanolamine + CO2 | - |
? | |
| phosphatidyl-L-serine | the enzyme catalyzes the final step in the biosynthesis of phosphatidylethanolamine | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36500, SDS-PAGE | Escherichia coli |
| dimer | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Escherichia coli |
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