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Literature summary for 4.1.1.63 extracted from
- Purification and characterization of an oxygen-sensitive reversible 3,4-dihydroxybenzoate decarboxylase from Clostridium hydroxybenzoicum (1996), J. Bacteriol., 178, 3539-3543.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3,4-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus |  |
| 3,4,5-Trihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
| Avidin | - |
Sedimentibacter hydroxybenzoicus | |
| HgCl2 | - |
Sedimentibacter hydroxybenzoicus | |
| hydroxylamine | - |
Sedimentibacter hydroxybenzoicus | |
| NaBH4 | - |
Sedimentibacter hydroxybenzoicus | |
| ZnSO4 | - |
Sedimentibacter hydroxybenzoicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
3,4-dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
5 * 57000, SDS-PAGE | Sedimentibacter hydroxybenzoicus |
| 270000 | - |
non-denaturing gradient PAGE | Sedimentibacter hydroxybenzoicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sedimentibacter hydroxybenzoicus | - |
Clostridium hydroxybenzoicum, JW/Z-1T | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen sensitive. 50% loss of activity after 10 min at 25°C, when exposed to air | Sedimentibacter hydroxybenzoicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sedimentibacter hydroxybenzoicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.6 | - |
- |
Sedimentibacter hydroxybenzoicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-Dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | Catechol + CO2 | - |
? | |
| Catechol + CO2 | - |
Sedimentibacter hydroxybenzoicus | 3,4-Dihydroxybenzoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentamer | 5 * 57000, SDS-PAGE | Sedimentibacter hydroxybenzoicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Sedimentibacter hydroxybenzoicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 11 | 50 | pH 7.0, relatively stable | Sedimentibacter hydroxybenzoicus |
| 25 | - |
pH 6.5-9.5, 2 h, stable | Sedimentibacter hydroxybenzoicus |
| 55 | - |
half-life: 1.5 h | Sedimentibacter hydroxybenzoicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 90 | - |
3,4-dihydroxybenzoate | - |
Sedimentibacter hydroxybenzoicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
under anaerobic conditions, both directions | Sedimentibacter hydroxybenzoicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | 25°C, 2 h, stable | Sedimentibacter hydroxybenzoicus |
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