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Literature summary for 4.1.1.61 extracted from

  • Lupa, B.; Lyon, D.; Shaw, L.N.; Sieprawska-Lupa, M.; Wiegel, J.
    Properties of the reversible nonoxidative vanillate/4-hydroxybenzoate decarboxylase from Bacillus subtilis (2008), Can. J. Microbiol., 54, 75-81.
    View publication on PubMed
Search for more literature for 4.1.1.61

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 4-hydroxybenzoate as substrate Km = 1 mmol/l Bacillus subtilis
additional information
-
 additional information vanillate as substrate Km = 4 mmol/l Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8569
-
 bsdD, calculated Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis C0H3U9 ATCC 6051
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
 uninduced, 4-hydroxybenzoate as substrate Bacillus subtilis
6
-
 3-methoxy-4-hydroxybenzoate-induced, 4-hydroxybenzoate as substrate Bacillus subtilis
11
-
 BsdCD, genes encoding the BsdCD are heterologously expressed in Escherichia coli from the plasmid pTrcHisC, 3-methoxy-4-hydroxybenzoate as substrate Bacillus subtilis
14
-
 BsdCD, genes encoding the BsdCD are heterologously expressed in Escherichia coli from the plasmid pTrcHisC, 4-hydroxybenzoate as substrate Bacillus subtilis
15
-
 uninduced, 3-methoxy-4-hydroxybenzoate as substrate Bacillus subtilis
16
-
 BsdBCD, genes encoding the BsdBCD are expressed in Escherichia coli from the plasmid pUC18, 3-methoxy-4-hydroxybenzoate as substrate Bacillus subtilis
23
-
 BsdBCD, genes encoding the BsdBCD are expressed in Escherichia coli from the plasmid pUC18, 4-hydroxybenzoate as substrate Bacillus subtilis
25
-
 3-methoxy-4-hydroxybenzoate-induced, 3-methoxy-4-hydroxybenzoate as substrate Bacillus subtilis
30
-
 4-hydroxybenzoate-induced, 3-methoxy-4-hydroxybenzoate as substrate Bacillus subtilis
31
-
 4-hydroxybenzoate-induced, 4-hydroxybenzoate as substrate Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methoxy-4-hydroxybenzoate vanillate Bacillus subtilis 2-methoxyphenol + CO2 guaiacol ?
4-Hydroxybenzoate
-
 Bacillus subtilis Phenol + CO2
-
 ?
additional information no reaction with: 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,6-dihydroxybenzoate, and 3,4-dihydroxybenzoate, 2,3,4-trihydroxybenzoate and 3,4,5-trihydroxybenzoate, 3,5-dihydroxy-2-naphthalene dicarboxylate, 2,8-dihydroxynaphthoate, 5-hydroxyisophthalate, 1-hydroxy-2-naphthoate, 2-hydroxy-1-naphthoate, 3-hydroxy-2-naphthoate, 6-hydroxynicotinate, 2-hydroxypyridine-3-carboxylate, 3-hydroxypyridine-2-carboxylate Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
4-hydroxybenzoate DC
-
 Bacillus subtilis
4-hydroxybenzoate decarboxylase
-
 Bacillus subtilis
vanillate/4-hydroxybenzoate decarboxylase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bacillus subtilis