Cloned (Comment) | Organism |
---|---|
gene PDC, recombinant expression in Escherichia coli strain HP-2, coexpression with genes TDC, TAL, SHT, and THT | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | genes PDC, TDC, TAL, SHT, and THT are introduced into engineered Escherichia coli strain HP-2 to synthesize N-(p-coumaroyl) phenethylamine and N-(p-coumaroyl) tyramine, method evaluation and optimization, overview. p-Coumaric acid is synthesized from tyrosine by TAL and phenethylamine is synthesized from phenylalanine by PDC. Therefore, the intracellular concentrations of both tyrosine and phenylalanine are critical for the final yield of N-(p-coumaroyl) phenethylamine | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | Pseudomonas putida | - |
phenylethylamine + CO2 | - |
? | |
L-phenylalanine | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | - |
phenylethylamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88JU5 | - |
- |
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | Q88JU5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Pseudomonas putida | phenylethylamine + CO2 | - |
? | |
L-phenylalanine | - |
Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | phenylethylamine + CO2 | - |
? | |
additional information | enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine | Pseudomonas putida | ? | - |
? | |
additional information | enzyme PDC from Pseudomonas putida is active on L-amino acids and shows higher substrate specificity for phenylalanine than for tyrosine | Pseudomonas putida ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 4.1.1.28 | Pseudomonas putida |
PDC | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |