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Literature summary for 4.1.1.51 extracted from

  • Mukherjee, T.; McCulloch, K.M.; Ealick, S.E.; Begley, T.P.
    Gene identification and structural characterization of the pyridoxal 5-phosphate degradative protein 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase from Mesorhizobium loti MAFF303099 (2007), Biochemistry, 46, 13606-13615.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Mesorhizobium loti

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, optimized crystallization conditions consist of 6-9% polyethylene glycol 8000 and 100 mM Tris buffer with a pH ranging from 7.0 to 7.5 Mesorhizobium loti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.366
-
3-hydroxy-2-methylpyridine-4,5-dicarboxylate in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol Mesorhizobium loti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
SDS-PAGE Mesorhizobium loti

Organism

Organism UniProt Comment Textmining
Mesorhizobium loti
-
-
-
Mesorhizobium loti MAFF303099
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity chromatography Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
-
Mesorhizobium loti 3-Hydroxy-2-methylpyridine-5-carboxylate + CO2
-
?
3-Hydroxy-2-methylpyridine-4,5-dicarboxylate
-
Mesorhizobium loti MAFF303099 3-Hydroxy-2-methylpyridine-5-carboxylate + CO2
-
?

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Mesorhizobium loti

Synonyms

Synonyms Comment Organism
3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase
-
Mesorhizobium loti
HMPDdc the enzyme is involved in the catabolism of pyridoxal 5'-phosphate Mesorhizobium loti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
3-hydroxy-2-methylpyridine-4,5-dicarboxylate in 100 mM Tris-HCl at pH 8.0 containing 100 mM NaCl and 2 mM dithiothreitol Mesorhizobium loti