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Literature summary for 4.1.1.50 extracted from

  • Kim, A.D.; Graham, D.E.; Seeholzer, S.H.; Markham, G.D.
    S-Adenosylmethionine decarboxylase from the archaeon Methanococcus jannaschii: identification of a novel family of pyruvoyl enzymes (2000), J. Bacteriol., 182, 6667-6672.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.50

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory are: MgCl2, CaCl2, EDTA, putrescine at 10 mM each, or KCl at 50 mM Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 S-adenosyl-L-methionine pH 7.4, 25°C Methanocaldococcus jannaschii
0.6
-
 S-adenosyl-L-methionine pH 7.4, 70°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no significant levels of metals Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6000
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
6793
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
6990
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
8000
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
30000
-
 PAGE Methanocaldococcus jannaschii
35000
-
 gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
after expression in Escherichia coli Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
 Methanocaldococcus jannaschii ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 3 h, full stability Methanocaldococcus jannaschii