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Literature summary for 4.1.1.49 extracted from

  • Sosa, M.H.; Giordana, L.; Nowicki, C.
    Exploring biochemical and functional features of Leishmania major phosphoenolpyruvate carboxykinase (2015), Arch. Biochem. Biophys., 583, 120-129 .
    View publication on PubMed
Search for more literature for 4.1.1.49

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure Leishmania major

Protein Variants

Protein Variants Comment Organism
D241A complete loss of activity Leishmania major
D242A complete loss of activity Leishmania major
H205Q mutation remarkably diminishes the activity, Km value for Mn2+ is 250fold increased Leishmania major
Y180A 10fold in crease in Km value of Mn2+ Leishmania major
Y180F 2- and 8fold decrease in specific acitvity for the oxaloacetate- and phosphoenolpyruvate-forming reactions, respectively Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 ATP wild-type, pH 7.4, 37°C Leishmania major
0.09
-
 ADP wild-type, pH 7.4, 37°C Leishmania major
0.09
-
 oxaloacetate wild-type, pH 7.4, 37°C Leishmania major
0.09
-
 ATP mutant Y180A, pH 7.4, 37°C Leishmania major
0.09
-
 ATP mutant Y180F, pH 7.4, 37°C Leishmania major
0.1
-
 ADP mutant Y180F, pH 7.4, 37°C Leishmania major
0.15
-
 ATP mutant H205Q, pH 7.4, 37°C Leishmania major
0.17
-
 oxaloacetate mutant Y180F, pH 7.4, 37°C Leishmania major
0.21
-
 oxaloacetate mutant H205Q, pH 7.4, 37°C Leishmania major
0.29
-
 ADP mutant Y180A, pH 7.4, 37°C Leishmania major
0.41
-
 ADP mutant H205Q, pH 7.4, 37°C Leishmania major
0.43
-
 oxaloacetate mutant Y180A, pH 7.4, 37°C Leishmania major
0.59
-
 phosphoenolpyruvate mutant Y180F, pH 7.4, 37°C Leishmania major
0.9
-
 phosphoenolpyruvate wild-type, pH 7.4, 37°C Leishmania major
1.24
-
 phosphoenolpyruvate mutant Y180A, pH 7.4, 37°C Leishmania major
1.63
-
 phosphoenolpyruvate mutant H205Q, pH 7.4, 37°C Leishmania major

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ may substitute for Mn2+ Leishmania major
Mn2+ preferred activator Leishmania major
additional information absolute requirement for divalent cations, the most effective activator is Mn2+. Cation preference in decreasing order is Mn2+ = Co2+ >> Zn2+ > Ni2+ > Mg2+. Ca2+ and Cu2+ are completely ineffective Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103000
-
 gel filtration Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major E9ADF9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51.6
-
 formation of phosphoenolpyruvate, pH 7.4, 37°C Leishmania major
69.6
-
 formation of oxaloacetate, pH 7.4, 37°C Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate + CO2
-
 Leishmania major ATP + oxaloacetate
-
 r
ATP + oxaloacetate
-
 Leishmania major ADP + phosphoenolpyruvate + CO2
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 59000, SDS-PAGE, recombinant His-tagged protein Leishmania major

Synonyms

Synonyms Comment Organism
LMJF_27_1810
-
 Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.67
-
 ADP mutant H205Q, pH 7.4, 37°C Leishmania major
0.7
-
 phosphoenolpyruvate mutant H205Q, pH 7.4, 37°C Leishmania major
2.66
-
 phosphoenolpyruvate mutant Y180A, pH 7.4, 37°C Leishmania major
3.18
-
 oxaloacetate mutant Y180A, pH 7.4, 37°C Leishmania major
3.22
-
 ADP mutant Y180A, pH 7.4, 37°C Leishmania major
3.86
-
 ATP mutant H205Q, pH 7.4, 37°C Leishmania major
3.93
-
 ATP mutant Y180A, pH 7.4, 37°C Leishmania major
4.41
-
 oxaloacetate mutant H205Q, pH 7.4, 37°C Leishmania major
6.75
-
 ATP mutant Y180F, pH 7.4, 37°C Leishmania major
9.07
-
 oxaloacetate mutant Y180F, pH 7.4, 37°C Leishmania major
29.8
-
 ADP mutant Y180F, pH 7.4, 37°C Leishmania major
37.78
-
 phosphoenolpyruvate mutant Y180F, pH 7.4, 37°C Leishmania major
48.09
-
 ATP wild-type, pH 7.4, 37°C Leishmania major
51.54
-
 oxaloacetate wild-type, pH 7.4, 37°C Leishmania major
65.52
-
 phosphoenolpyruvate wild-type, pH 7.4, 37°C Leishmania major
78.82
-
 ADP wild-type, pH 7.4, 37°C Leishmania major

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.43
-
 phosphoenolpyruvate mutant H205Q, pH 7.4, 37°C Leishmania major
1.63
-
 ADP mutant H205Q, pH 7.4, 37°C Leishmania major
2.15
-
 phosphoenolpyruvate mutant Y180A, pH 7.4, 37°C Leishmania major
7.4
-
 oxaloacetate mutant Y180A, pH 7.4, 37°C Leishmania major
11.1
-
 ADP mutant Y180A, pH 7.4, 37°C Leishmania major
21
-
 oxaloacetate mutant H205Q, pH 7.4, 37°C Leishmania major
26.3
-
 ATP mutant H205Q, pH 7.4, 37°C Leishmania major
42.3
-
 ATP mutant Y180A, pH 7.4, 37°C Leishmania major
54.6
-
 oxaloacetate mutant Y180F, pH 7.4, 37°C Leishmania major
63.6
-
 phosphoenolpyruvate mutant Y180F, pH 7.4, 37°C Leishmania major
72
-
 phosphoenolpyruvate wild-type, pH 7.4, 37°C Leishmania major
72.6
-
 ATP mutant Y180F, pH 7.4, 37°C Leishmania major
304
-
 ADP mutant Y180F, pH 7.4, 37°C Leishmania major
573
-
 oxaloacetate wild-type, pH 7.4, 37°C Leishmania major
876
-
 ADP wild-type, pH 7.4, 37°C Leishmania major
891
-
 ATP wild-type, pH 7.4, 37°C Leishmania major