Crystallization (Comment) | Organism |
---|---|
molecular modeling of structure | Leishmania major |
Protein Variants | Comment | Organism |
---|---|---|
D241A | complete loss of activity | Leishmania major |
D242A | complete loss of activity | Leishmania major |
H205Q | mutation remarkably diminishes the activity, Km value for Mn2+ is 250fold increased | Leishmania major |
Y180A | 10fold in crease in Km value of Mn2+ | Leishmania major |
Y180F | 2- and 8fold decrease in specific acitvity for the oxaloacetate- and phosphoenolpyruvate-forming reactions, respectively | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
ATP | wild-type, pH 7.4, 37°C | Leishmania major | |
0.09 | - |
ADP | wild-type, pH 7.4, 37°C | Leishmania major | |
0.09 | - |
oxaloacetate | wild-type, pH 7.4, 37°C | Leishmania major | |
0.09 | - |
ATP | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
0.09 | - |
ATP | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
0.1 | - |
ADP | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
0.15 | - |
ATP | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
0.17 | - |
oxaloacetate | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
0.21 | - |
oxaloacetate | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
0.29 | - |
ADP | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
0.41 | - |
ADP | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
0.43 | - |
oxaloacetate | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
0.59 | - |
phosphoenolpyruvate | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
0.9 | - |
phosphoenolpyruvate | wild-type, pH 7.4, 37°C | Leishmania major | |
1.24 | - |
phosphoenolpyruvate | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
1.63 | - |
phosphoenolpyruvate | mutant H205Q, pH 7.4, 37°C | Leishmania major |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | may substitute for Mn2+ | Leishmania major | |
Mn2+ | preferred activator | Leishmania major | |
additional information | absolute requirement for divalent cations, the most effective activator is Mn2+. Cation preference in decreasing order is Mn2+ = Co2+ >> Zn2+ > Ni2+ > Mg2+. Ca2+ and Cu2+ are completely ineffective | Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103000 | - |
gel filtration | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | E9ADF9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
51.6 | - |
formation of phosphoenolpyruvate, pH 7.4, 37°C | Leishmania major |
69.6 | - |
formation of oxaloacetate, pH 7.4, 37°C | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate + CO2 | - |
Leishmania major | ATP + oxaloacetate | - |
r | |
ATP + oxaloacetate | - |
Leishmania major | ADP + phosphoenolpyruvate + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59000, SDS-PAGE, recombinant His-tagged protein | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
LMJF_27_1810 | - |
Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
ADP | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
0.7 | - |
phosphoenolpyruvate | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
2.66 | - |
phosphoenolpyruvate | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
3.18 | - |
oxaloacetate | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
3.22 | - |
ADP | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
3.86 | - |
ATP | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
3.93 | - |
ATP | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
4.41 | - |
oxaloacetate | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
6.75 | - |
ATP | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
9.07 | - |
oxaloacetate | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
29.8 | - |
ADP | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
37.78 | - |
phosphoenolpyruvate | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
48.09 | - |
ATP | wild-type, pH 7.4, 37°C | Leishmania major | |
51.54 | - |
oxaloacetate | wild-type, pH 7.4, 37°C | Leishmania major | |
65.52 | - |
phosphoenolpyruvate | wild-type, pH 7.4, 37°C | Leishmania major | |
78.82 | - |
ADP | wild-type, pH 7.4, 37°C | Leishmania major |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
phosphoenolpyruvate | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
1.63 | - |
ADP | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
2.15 | - |
phosphoenolpyruvate | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
7.4 | - |
oxaloacetate | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
11.1 | - |
ADP | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
21 | - |
oxaloacetate | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
26.3 | - |
ATP | mutant H205Q, pH 7.4, 37°C | Leishmania major | |
42.3 | - |
ATP | mutant Y180A, pH 7.4, 37°C | Leishmania major | |
54.6 | - |
oxaloacetate | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
63.6 | - |
phosphoenolpyruvate | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
72 | - |
phosphoenolpyruvate | wild-type, pH 7.4, 37°C | Leishmania major | |
72.6 | - |
ATP | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
304 | - |
ADP | mutant Y180F, pH 7.4, 37°C | Leishmania major | |
573 | - |
oxaloacetate | wild-type, pH 7.4, 37°C | Leishmania major | |
876 | - |
ADP | wild-type, pH 7.4, 37°C | Leishmania major | |
891 | - |
ATP | wild-type, pH 7.4, 37°C | Leishmania major |